The Antihypertensive Potential of Aqueous extract of Peristrophe Bivalvis (L.) Merr. is via Up-regulation of Cyclic Guanosine Monophosphate and Down-regulation of the Renin-angiotensin System.

Esther Oluwasola Aluko, Ubong Edem David, Abodunrin Adebayo Ojetola, Adesoji Adedipe Fasanmade
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Abstract

Background: Hypertension is a major risk factor for cardiovascular diseases. Peristrophe bivalvis (PB) is used for the treatment of hypertension, painful sprains, skin diseases, tuberculosis, acute bronchitis, anti-inflammatory conditions, hepatitis, and diabetes. Its antihypertensive potential has been investigated and documented. This study investigated the antihypertensive mechanism of aqueous extract of PB leaf (APB) on L-NAME-induced hypertension.

Methods: Thirty male wistar rats (150-170 g) were grouped into five (n=5). Group 1 received 10 mL/kg of distilled water (control), while groups 2-5 were administered 60 mg/kg of L-NAME (L-NAME60) orally for eight weeks to induce hypertension. After eight weeks, groups 2-5 received L-NAME60+distilled water (HNT), distilled water (HRE), L-NAME60+APB (200 mg/kg, [HAPB]), and L-NAME60+ramipril (10 mg/kg, [HRA]), respectively, for five weeks. The BP was measured by the tail-cuff method. The blood sample was obtained under anesthesia, and tissue samples were obtained after euthanasia. Serum renin, ACE, angiotensin-II, endothelin-1, and cyclic guanosine monophosphate (cGMP) levels were measured using ELISA techniques. Malondialdehyde, superoxide dismutase (SOD), and reduced glutathione (GSH) levels were measured by spectrophotometry. Data were analyzed using ANOVA at α0.05.

Results: The BP significantly decreased in HAPB compared to HNT. Renin, ACE, and angiotensin- II levels significantly decreased while cGMP levels increased in the HAPB group compared to HNT. Malondialdehyde levels significantly decreased, and SOD and GSH levels increased compared to HNT.

Conclusion: Peristrophe bivalvis aqueous leaf extract reduced blood pressure in hypertensive rats by modulating the cGMP signalling pathway and the renin-angiotensin system.

Peristrophe Bivalvis (L.) Merr.水提取物通过上调单磷酸环鸟苷和下调肾素-血管紧张素系统发挥抗高血压作用
背景:高血压是心血管疾病的主要风险因素:高血压是心血管疾病的主要危险因素。双壳贝母(PB)可用于治疗高血压、扭伤疼痛、皮肤病、肺结核、急性支气管炎、消炎、肝炎和糖尿病。其抗高血压的潜力已得到研究和记录。本研究探讨了枸杞叶水提取物(APB)对 L-NAME 引起的高血压的降压机制:方法:30 只雄性 Wistar 大鼠(150-170 克)分为 5 组(n=5)。第1组接受10毫升/千克蒸馏水(对照组),第2-5组口服60毫克/千克L-NAME(L-- NAME60)诱导高血压八周。八周后,2-5组分别接受L-NAME60+蒸馏水(HNT)、蒸馏水(HRE)、L-NAME60+APB(200毫克/千克,[HAPB])和L-NAME60+雷米普利(10毫克/千克,[HRA])治疗五周。血压测量采用尾袖法。血液样本在麻醉状态下采集,组织样本在安乐死后采集。使用 ELISA 技术测量血清肾素、ACE、血管紧张素-II、内皮素-1 和环磷酸鸟苷(cGMP)的水平。丙二醛、超氧化物歧化酶(SOD)和还原型谷胱甘肽(GSH)水平采用分光光度法进行测量。数据采用方差分析,α0.05:结果:与 HNT 相比,HAPB 的血压明显下降。与 HNT 相比,HAPB 组的肾素、ACE 和血管紧张素 II 水平明显下降,而 cGMP 水平上升。与 HNT 相比,丙二醛水平明显下降,SOD 和 GSH 水平上升:结论:双壳贝叶水提取物通过调节 cGMP 信号通路和肾素-血管紧张素系统降低了高血压大鼠的血压。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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