miRNA-mediated insect-resistant transgenic rice poses no risk to a non-target parasitoid, Cotesia chilonis, via direct feeding or through its target host.

IF 2.9 1区 农林科学 Q1 ENTOMOLOGY
Cong Dang, Shan Xiao, Fang Wang, Qi Fang, Hongwei Yao, Kang He, Fei Li, Dawei Xue, Gongyin Ye
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引用次数: 0

Abstract

MicroRNAs (miRNAs) have started to play an important role in pest control, and novel miRNA-based transgenic insect-resistant plants are now emerging. However, an environmental risk assessment of these novel transgenic plants expressing insect miRNAs must be undertaken before promoting their application. Here, transgenic miR-14 rice, which has high resistance to the rice stem borer Chilo suppressalis, was used as an example for evaluation in this study. Taking the tier 1 risk assessment method in Bacillus thuringiensis (Bt) crops as a reference, the effects of the direct exposure of a non-target parasitoid, Cotesia chilonis, to a high concentration of miRNA were evaluated. The results showed that direct feeding with miR-14 at high concentration had no significant effects on the biological parameters of Co. chilonis, whereas when miR-14 was injected into Ch. suppressalis-parasitized larvae, the development duration of Co. chilonis was significantly affected. In combination with the real conditions of the rice paddy field, it could be inferred that transgenic miR-14 rice has no significant negative effects on the important non-target parasitoid, Co. chilonis. These results will provide a foundation for the establishment of a new safety evaluation system for novel RNAi-based transgenic plants.

miRNA 介导的抗虫转基因水稻通过直接取食或目标寄主对非目标寄生虫 Cotesia chilonis 不构成风险。
微小核糖核酸(miRNA)已开始在害虫控制中发挥重要作用,基于 miRNA 的新型转基因抗虫植物也正在出现。然而,在推广应用之前,必须对这些表达昆虫 miRNA 的新型转基因植物进行环境风险评估。本研究以对水稻二化螟(Chilo suppressalis)具有高抗性的转基因 miR-14 水稻为例进行评估。以苏云金芽孢杆菌(Bt)作物的一级风险评估方法为参考,评估了非目标寄生虫 Cotesia chilonis 直接接触高浓度 miRNA 的影响。结果表明,直接喂食高浓度的 miR-14 对 Cotesia chilonis 的生物参数没有显著影响,而将 miR-14 注入被 Ch. suppressalis 寄生的幼虫体内,Cotesia chilonis 的发育持续时间会受到显著影响。结合稻田的实际情况,可以推断转基因 miR-14 水稻对重要的非靶标寄生虫 Chilonis 没有明显的负面影响。这些结果将为建立基于 RNAi 的新型转基因植物安全评价体系奠定基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Insect Science
Insect Science 生物-昆虫学
CiteScore
7.80
自引率
5.00%
发文量
1379
审稿时长
6.0 months
期刊介绍: Insect Science is an English-language journal, which publishes original research articles dealing with all fields of research in into insects and other terrestrial arthropods. Papers in any of the following fields will be considered: ecology, behavior, biogeography, physiology, biochemistry, sociobiology, phylogeny, pest management, and exotic incursions. The emphasis of the journal is on the adaptation and evolutionary biology of insects from the molecular to the ecosystem level. Reviews, mini reviews and letters to the editor, book reviews, and information about academic activities of the society are also published.
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