Functionalized Protein Binders in Developmental Biology.

IF 11.4 1区 生物学 Q1 CELL BIOLOGY
Sophie T Schnider, M Alessandra Vigano, Markus Affolter, Gustavo Aguilar
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引用次数: 0

Abstract

Developmental biology has greatly profited from genetic and reverse genetic approaches to indirectly studying protein function. More recently, nanobodies and other protein binders derived from different synthetic scaffolds have been used to directly dissect protein function. Protein binders have been fused to functional domains, such as to lead to protein degradation, relocalization, visualization, or posttranslational modification of the target protein upon binding. The use of such functionalized protein binders has allowed the study of the proteome during development in an unprecedented manner. In the coming years, the advent of the computational design of protein binders, together with further advances in scaffold engineering and synthetic biology, will fuel the development of novel protein binder-based technologies. Studying the proteome with increased precision will contribute to a better understanding of the immense molecular complexities hidden in each step along the way to generate form and function during development.

发育生物学中的功能化蛋白质粘合剂。
发育生物学从间接研究蛋白质功能的遗传和反向遗传方法中获益匪浅。最近,从不同合成支架中提取的纳米抗体和其他蛋白质结合剂被用于直接剖析蛋白质功能。蛋白质结合体已与功能域融合,如在结合后导致蛋白质降解、重新定位、可视化或对目标蛋白质进行翻译后修饰。这种功能化蛋白质结合剂的使用使人们能够以前所未有的方式研究发育过程中的蛋白质组。未来几年,蛋白质结合剂计算设计的出现,以及支架工程和合成生物学的进一步发展,将推动基于蛋白质结合剂的新型技术的发展。更精确地研究蛋白质组将有助于更好地理解发育过程中产生形态和功能的每一步所隐藏的巨大分子复杂性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
19.50
自引率
0.00%
发文量
21
期刊介绍: The Annual Review of Cell and Developmental Biology, established in 1985, comprehensively addresses major advancements in cell and developmental biology. Encompassing the structure, function, and organization of cells, as well as the development and evolution of cells in relation to both single and multicellular organisms, the journal explores models and tools of molecular biology. As of the current volume, the journal has transitioned from gated to open access through Annual Reviews' Subscribe to Open program, making all articles published under a CC BY license.
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