{"title":"A multi-biocatalytic system for effective fumarate synthesis from pyruvate and gaseous CO2†","authors":"Mika Takeuchi and Yutaka Amao","doi":"10.1039/D3SU00486D","DOIUrl":null,"url":null,"abstract":"<p >Fumarate, an unsaturated dicarboxylic acid, is an important material for producing unsaturated polyester resins and biodegradable plastics. Fumarate synthesis from petroleum-derived benzene and butane as starting materials is expected to be replaced by synthesis methods from renewable raw materials. In this work, fumarate synthesis from gaseous CO<small><sub>2</sub></small> and pyruvate in an aqueous medium using a multi-biocatalytic system consisting of pyruvate carboxylase (PC), malate dehydrogenase (MDH) and fumarase (FUM) in the presence of ATP and NADH is accomplished. The conversion yield of fumarate from pyruvate using this system was estimated to be approximately 16% after 5 h of incubation.</p>","PeriodicalId":74745,"journal":{"name":"RSC sustainability","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/su/d3su00486d?page=search","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"RSC sustainability","FirstCategoryId":"1085","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/su/d3su00486d","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Fumarate, an unsaturated dicarboxylic acid, is an important material for producing unsaturated polyester resins and biodegradable plastics. Fumarate synthesis from petroleum-derived benzene and butane as starting materials is expected to be replaced by synthesis methods from renewable raw materials. In this work, fumarate synthesis from gaseous CO2 and pyruvate in an aqueous medium using a multi-biocatalytic system consisting of pyruvate carboxylase (PC), malate dehydrogenase (MDH) and fumarase (FUM) in the presence of ATP and NADH is accomplished. The conversion yield of fumarate from pyruvate using this system was estimated to be approximately 16% after 5 h of incubation.
不饱和二羧酸富马酸盐是生产不饱和聚酯树脂和生物降解塑料的重要原料。以源自石油的苯和丁烷为起始原料合成的富马酸有望被可再生原料合成法所取代。本研究利用由丙酮酸羧化酶（PC）、苹果酸脱氢酶（MDH）和富马酸酶（FUM）组成的多重生物催化系统，在 ATP 和 NADH 的存在下，在水介质中从气态 CO2 和丙酮酸合成富马酸。据估计，在培养 5 小时后，使用该系统将丙酮酸转化为富马酸的转化率约为 16%。
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