Cellular response to plasma-generated electrical and chemical stimulation of equal electricity

IF 3.1 3区 物理与天体物理 Q2 PHYSICS, APPLIED
Airi Nakayama, Siwei Liu, Ken-ichi Yano, Tomoki Nakajima and Takehiko Sato
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引用次数: 0

Abstract

Plasma medicine is a novel field of research that has the potential in life sciences, including cancer treatment. It has been believed that all of these potential applications depend on the chemical factor of the plasma. However, the electrical factors that are produced simultaneously with the chemical factors have not been researched thoroughly. To assess the effect of plasma on the response of cells, a system that enables simultaneous comparison of the effects of chemical and electrical factors of plasma with equal electricity was invented. This system separates the polarity of plasma and isolates the chemical species from the electric stimulation. Using this system, HT-1080 cells were exposed to plasma for 10 min, 1 h, and 24 h. The 10 min plasma treatment showed a clear difference in the polarity of plasma, where cells under exposure to positive plasma died while cells in other conditions survived. An hour of plasma treatment affected the cells under the negative plasma, where the cell viability was reduced to half. Meanwhile, the electric stimulation did not affect the cell viability but did alter the cell membrane. Collectively, this study demonstrates the differential effects of three factors (electric factor, positive plasma, negative plasma) under the same condition: both the electrical and chemical effects of plasma-generated stimuli with equal amounts of electricity were successfully observed.
细胞对等离子体产生的等量电刺激和化学刺激的反应
等离子体医学是一个新的研究领域,在生命科学(包括癌症治疗)方面具有潜力。人们一直认为,所有这些潜在应用都取决于等离子体的化学因素。然而,与化学因子同时产生的电因子尚未得到深入研究。为了评估等离子体对细胞反应的影响,我们发明了一种系统,可以同时比较等离子体化学因子和等电因子的影响。该系统分离了等离子体的极性,并将化学物质与电刺激隔离开来。使用该系统将 HT-1080 细胞暴露在等离子体中 10 分钟、1 小时和 24 小时。10 分钟的等离子体处理显示出等离子体极性的明显差异,暴露在正等离子体中的细胞死亡,而其他条件下的细胞存活。一小时的等离子体处理影响了负等离子体下的细胞,细胞存活率降低了一半。同时,电刺激不会影响细胞的存活率,但会改变细胞膜。总之,这项研究证明了三种因素(电因子、正等离子体、负等离子体)在相同条件下的不同影响:成功地观察到了等离子体产生的等量电刺激的电效应和化学效应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Physics D: Applied Physics
Journal of Physics D: Applied Physics 物理-物理:应用
CiteScore
6.80
自引率
8.80%
发文量
835
审稿时长
2.1 months
期刊介绍: This journal is concerned with all aspects of applied physics research, from biophysics, magnetism, plasmas and semiconductors to the structure and properties of matter.
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