Screening of botanical spatial mosquito repellents from 64 essential oils and their interactions with odorant‐binding protein‐3

IF 1.7 3区农林科学 Q2 ENTOMOLOGY

Journal of Applied Entomology Pub Date : 2024-07-18 DOI:10.1111/jen.13330

Xiaofei Ren, Wenjiao Li, Jinmiao Zhang, Yingxiang Zhu, Xiaohui Hou, Hanwen Miao, Ximing Xu, Dongjing Zhang, Zhaojun Sheng

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引用次数: 0

Abstract

Personal protection from mosquito bites is essential for preventing mosquito‐borne diseases. Mosquito repellents are effective methods for preventing mosquito bites. Due to their ease of use and indirect skin contact, mosquito‐repellent stickers loaded with essential oils (EOs) have recently become favoured as novel spatial repellents. To discover active EOs with high spatial repellency, 64 commercial EOs were screened using a modified arm‐in‐cage assay. Four EOs, including nutmeg, melissa, eucalyptus and cinnamon, displayed longer complete protection times (CPTs) of about 60 min. Ten main constituents from the four active EOs were identified by gas chromatography–mass spectrometry (GC–MS), and their spatial repellent activities were further evaluated. 1,8‐Cineole, cinnamaldehyde and citral showed potent repellent activity, with average CPTs of 67.5, 63.8 and 60 min respectively. The interactions of 1,8‐cineole and citral with odorant‐binding protein‐3 (OBP‐3) were studied via molecular docking. Both compounds are docked well in the high hydrophobic pocket and interacted with AealOBP3 through Van der Waals interactions with Phe105, Phe112, Leu114, Met48 and Val50. These natural products provide promising lead fragments for the further development of botanical spatial repellents.

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从 64 种精油中筛选植物空间驱蚊剂及其与气味结合蛋白-3 的相互作用

防止蚊虫叮咬的个人防护对于预防蚊媒疾病至关重要。驱蚊剂是预防蚊虫叮咬的有效方法。由于使用方便且可间接接触皮肤，含有精油（EOs）的驱蚊贴作为新型空间驱蚊剂近来备受青睐。为了发现具有高空间驱避性的活性 EO，我们使用改良的笼中臂试验筛选了 64 种商用 EO。肉豆蔻、香紫苏、桉树和肉桂等四种 EO 显示出较长的完全保护时间（CPT），约为 60 分钟。通过气相色谱-质谱法（GC-MS）鉴定了四种活性环氧乙烷中的十种主要成分，并进一步评估了它们的空间驱避活性。1,8-丁烯、肉桂醛和柠檬醛显示出了强大的驱避活性，其平均 CPTs 分别为 67.5、63.8 和 60 分钟。通过分子对接研究了 1,8-丁烯和柠檬醛与气味结合蛋白-3（OBP-3）的相互作用。这两种化合物在高疏水口袋中对接良好，并通过与 Phe105、Phe112、Leu114、Met48 和 Val50 的范德华相互作用与 AealOBP3 发生相互作用。这些天然产物为进一步开发植物空间驱避剂提供了前景广阔的先导片段。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Entomology 生物-昆虫学

CiteScore

3.40

自引率

5.30%

发文量

132

审稿时长

6 months

期刊介绍： The Journal of Applied Entomology publishes original articles on current research in applied entomology, including mites and spiders in terrestrial ecosystems. Submit your next manuscript for rapid publication: the average time is currently 6 months from submission to publication. With Journal of Applied Entomology''s dynamic article-by-article publication process, Early View, fully peer-reviewed and type-set articles are published online as soon as they complete, without waiting for full issue compilation.