Daixi Xie, Bingda Chen, Yonggan Xue, Zhiyuan Sun, Bobin Ning, Zeying Zhang, Jimei Chi, Meng Su, Yanlin Song
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引用次数: 0
Abstract
Organoid biochips can replicate the micro-environment and functional traits of human organs in vitro, reflecting the physiological and pathological features of the human body. It provides a new platform for disease modeling and drug screening. However, the manual process of organoid cultivation and biochip construction using decellularized extracellular matrix-based gel is typically complex, expensive, and time-consuming (at least one month), which significantly hinders practical application. Here, we introduce a micro-needle-based pneumatic printing strategy for residual-free and high-throughput construction of patient-derived organoid biochips. By developing printable and biomimetic hydrogels, biopsy samples of cancer tissues can be effectively processed into discrete cells. Patient-derived colorectal cancer (CRC) cells in carboxymethylcellulose (CMC) and sodium alginate modified by adhesion sites exhibit high viability at 92%. Through a microneedle, the cell-ink utilization exceeds 90%. Especially, the organoid biochips can effectively be fabricated, and single cells in biochips can proliferate and differentiate into organoids with typical morphology. Finally, the patient-derived CRC organoids are used as the biochips for drug testing, which give the personalized drug screening information in a week. Overall, through the microprinting strategy and biomimetic hydrogels, the utilization rate of cells and the construction efficiency of organoid chips can be improved. This work provides a new approach for high-throughput printing patient-derived organoid biochips in precision medicine.
期刊介绍:
Science China Materials (SCM) is a globally peer-reviewed journal that covers all facets of materials science. It is supervised by the Chinese Academy of Sciences and co-sponsored by the Chinese Academy of Sciences and the National Natural Science Foundation of China. The journal is jointly published monthly in both printed and electronic forms by Science China Press and Springer. The aim of SCM is to encourage communication of high-quality, innovative research results at the cutting-edge interface of materials science with chemistry, physics, biology, and engineering. It focuses on breakthroughs from around the world and aims to become a world-leading academic journal for materials science.