Magnesium-doped biochars increase soil phosphorus availability by regulating phosphorus retention, microbial solubilization and mineralization

Abstract

Despite fertilization efforts, phosphorus (P) availability in soils remains a major constraint to global plant productivity. Soil incorporation of biochar could promote soil P availability but its effects remain uncertain. To attain further improvements in soil P availability with biochar, we developed, characterized, and evaluated magnesium-oxide (MgO) and sepiolite (Mg₄Si₆O₁₅(OH)₂·6H₂O)-functionalized biochars with optimized P retention/release capacity. Field-based application of these biochars for improving P availability and their mechanisms during three growth stages of maize was investigated. We further leveraged next-generation sequencing to unravel their impacts on the plant growth-stage shifts in soil functional genes regulating P availability. Results showed insignificant variation in P availability between single super phosphate fertilization (F) and its combination with raw biochar (BF). However, the occurrence of Mg-bound minerals on the optimized biochars’ surface adjusted its surface charges and properties and improved the retention and slow release of inorganic P. Compared to BF, available P (AP) was 26.5% and 19.1% higher during the 12-leaf stage and blister stage, respectively, under MgO-optimized biochar + F treatment (MgOBF), and 15.5% higher under sepiolite-biochar + F (SBF) during maize physiological maturity. Cumulatively, AP was 15.6% and 13.2% higher in MgOBF and SBF relative to BF. Hence, plant biomass, grain yield, and P uptake were highest in MgOBF and SBF, respectively at harvest. Optimized-biochar amendment stimulated microbial 16SrRNA gene diversity and suppressed the expression of P starvation response and P uptake and transport-related genes while stimulating P solubilization and mineralization genes. Thus, the optimized biochars promoted P availability via the combined processes of slow-release of retained phosphates, while inducing the microbial solubilization and mineralization of inorganic and organic P, respectively. Our study advances strategies for reducing cropland P limitation and reveals the potential of optimized biochars for improving P availability on the field scale.

Graphical Abstract