Probiotic potential of autochthonous Lactobacillus species from buffalo calves in controlling multidrug resistant Escherichia coli.

IF 0.8 4区农林科学 Q3 ZOOLOGY

Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-06-15 DOI:10.30466/vrf.2024.2012559.4024

Srivani Moturi, Lakshmi Kavitha Kommalapati, Muralidhar Metta, Venkata Seshaiah Chappidi, Srilakshmi Jatavathu

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Abstract

The aim of this study was to investigate the probiotic potential of autochthonous Lactobacillus species isolated from buffalo calves against multidrug-resistant Escherichia coli. A total of 252 rectal swabs were collected from healthy neonatal buffalo calves under 30 days old from six districts of Andhra Pradesh, India in a completely randomized design from August 2019 to August 2021, of which 190 Lactobacillus strains were isolated based on cultural, morphological, biochemical and molecular tests. Out of 190 isolates, 57 showed high levels of auto-aggregation (> 80.00%) and hydrophobicity (> 60.00%) and 51 of the 57 isolates had a zone of inhibition greater than 15.00 mm in diameter against multidrug-resistant E. coli in an Agar well diffusion assay. Among the 51 isolates, 36 were found to be acid and bile tolerant and showed varying levels of sensitivity to antibiotics such as erythromycin, clindamycin, tetracycline, chloramphenicol, and ampicillin. Among the 36 isolates, Limosilactobacillus reuteri 178, L. reuteri 209, L. fermentum 182, L. fermentum 211, and Lactiplanti-bacillus plantarum 34 were non-hemolytic, and none of the isolates were able to hydrolyse gelatine. Therefore, these five autochthonous Lactobacillus species may be used in probiotic or synbiotic formulations against multidrug resistant E. coli in buffalo calves.

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水牛犊自生乳酸杆菌在控制耐多药大肠杆菌方面的益生潜力

本研究旨在调查从水牛犊牛体内分离出的自生乳酸杆菌对耐多药大肠杆菌的益生潜力。研究采用完全随机设计的方法，从 2019 年 8 月至 2021 年 8 月期间，从印度安得拉邦 6 个地区 30 天以下的健康新生水牛犊牛身上共采集了 252 份直肠拭子，并根据培养、形态、生化和分子检测结果分离出 190 株乳酸杆菌。在 190 株分离菌株中，有 57 株表现出高度的自动聚集性（> 80.00%）和疏水性（> 60.00%），在琼脂井扩散试验中，57 株分离菌株中有 51 株对耐多药大肠杆菌的抑制区直径大于 15.00 毫米。在这 51 个分离物中，发现有 36 个耐酸和耐胆汁，并对红霉素、林可霉素、四环素、氯霉素和氨苄西林等抗生素表现出不同程度的敏感性。在 36 个分离菌株中，Limosilactobacillus reuteri 178、L. reuteri 209、L. fermentum 182、L. fermentum 211 和 Lactiplanti-bacillus plantarum 34 不溶血，且没有一个分离菌株能水解明胶。因此，这五种自生乳杆菌可用于益生菌或合成益生菌配方，以对抗水产犊牛体内的耐多药大肠杆菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Research Forum Veterinary-General Veterinary

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： Veterinary Research Forum (VRF) is a quarterly international journal committed to publish worldwide contributions on all aspects of veterinary science and medicine, including anatomy and histology, physiology and pharmacology, anatomic and clinical pathology, parasitology, microbiology, immunology and epidemiology, food hygiene, poultry science, fish and aquaculture, anesthesia and surgery, large and small animal internal medicine, large and small animal reproduction, biotechnology and diagnostic imaging of domestic, companion and farm animals.