Advanced screening methods for assessing motility and hatching in plant-parasitic nematodes.

IF 4.7 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Plant Methods Pub Date : 2024-07-20 DOI:10.1186/s13007-024-01233-z

Alena Kadlecová, Romana Hendrychová, Tomáš Jirsa, Václav Čermák, Mengmeng Huang, Florian M W Grundler, A Sylvia S Schleker

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引用次数: 0

Abstract

Background: Plant-parasitic nematodes are economically important pests responsible for substantial losses in agriculture. Researchers focusing on plant-parasitic nematodes, especially on finding new ways of their control, often need to assess basic parameters such as their motility, viability, and reproduction. Traditionally, these assays involve visually counting juveniles and eggs under a dissecting microscope, making this investigation time-consuming and laborious.

Results: In this study, we established a procedure to efficiently determine the motility of two plant-parasitic nematode species, Heterodera schachtii and Ditylenchus destructor, using the WMicrotracker ONE platform. Additionally, we demonstrated that hatching of the cyst nematode H. schachtii can be evaluated using both the WMicrotracker ONE and by assessing the enzymatic activity of chitinase produced during hatching.

Conclusions: We present fast and straightforward protocols for studying nematode motility and hatching that allow us to draw conclusions about viability and survival. Thus, these methods are useful tools for facilitating fast and efficient evaluation in various fields of research focused on plant-parasitic nematodes.

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评估植物寄生线虫运动和孵化的先进筛选方法。

背景：植物寄生线虫是对农业造成重大损失的重要经济害虫。关注植物寄生线虫的研究人员，尤其是寻找控制线虫的新方法的研究人员，经常需要评估线虫的运动、活力和繁殖等基本参数。传统上，这些检测需要在解剖显微镜下目测幼虫和虫卵的数量，因此这种调查既费时又费力：在这项研究中，我们建立了一套程序，利用 WMicrotracker ONE 平台有效测定两种植物寄生线虫--Heterodera schachtii 和 Ditylenchus destructor 的运动能力。此外，我们还证明了可以利用 WMicrotracker ONE 和评估孵化过程中产生的几丁质酶的酶活性来评估 H. schachtii 囊线虫的孵化情况：结论：我们提出了研究线虫运动和孵化的快速而直接的方案，使我们能够得出有关生存能力和存活率的结论。因此，这些方法是促进植物寄生线虫各研究领域快速高效评估的有用工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Methods 生物-植物科学

CiteScore

9.20

自引率

3.90%

发文量

121

审稿时长

2 months

期刊介绍： Plant Methods is an open access, peer-reviewed, online journal for the plant research community that encompasses all aspects of technological innovation in the plant sciences. There is no doubt that we have entered an exciting new era in plant biology. The completion of the Arabidopsis genome sequence, and the rapid progress being made in other plant genomics projects are providing unparalleled opportunities for progress in all areas of plant science. Nevertheless, enormous challenges lie ahead if we are to understand the function of every gene in the genome, and how the individual parts work together to make the whole organism. Achieving these goals will require an unprecedented collaborative effort, combining high-throughput, system-wide technologies with more focused approaches that integrate traditional disciplines such as cell biology, biochemistry and molecular genetics. Technological innovation is probably the most important catalyst for progress in any scientific discipline. Plant Methods’ goal is to stimulate the development and adoption of new and improved techniques and research tools and, where appropriate, to promote consistency of methodologies for better integration of data from different laboratories.