{"title":"Effects of <i>Laurus Nobilis</i> Eye Drop on Selenite-Induced Cataract Formation and Oxidative Stress-Related Parameters in Rabbits: An Experimental Study.","authors":"Marisa Palazzo, Marina Concilio, Luigi Ambrosone, Michele Rinaldi, Fausto Tranfa, Ciro Costagliola","doi":"10.1080/02713683.2024.2380440","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the protective role of <i>Laurus Nobilis</i> eye drops on selenite-induced cataracts in suckling rabbits.</p><p><strong>Methods: </strong>Fifteen male albino suckling rabbits with no signs of ocular inflammation were randomly assigned to three groups: controls (Group A), sodium-selenite group (Group B) and sodium-selenite plus <i>Laurus Nobilis</i> group (Group C). By selenite treatment, cataract formation was experimentally induced and then graded. The grade of oxidative stress was defined in the lens, measuring the concentration of malondialdehyde, alpha-tocopherol, oxidized glutathione, ascorbic acid and hydrogen peroxide, and in blood samples as levels of alpha-tocopherol and malondialdehyde.</p><p><strong>Results: </strong>Mean lens concentrations of GSSG, H2O2, and MDA levels in group B were significantly higher than in both group C and control. Ascorbic acid and alpha-tocopherol concentrations were lower in group B than in both group C and A. As plasma oxidative status markers, the level of MDA was higher in group B respected group C and A. The mean alpha-tocopherol levels in group B were significantly lower than in both group A and group C.</p><p><strong>Conclusions: </strong>In animals treated with <i>Laurus Nobilis</i>-based eye drops, inflammation was inhibited, and lipid peroxidation was significantly reduced. <i>Laurus nobilis</i> leaves extract represents a good source of antioxidant components that may contrast sodium selenite-induced cataractogenesis in suckling rabbits.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1247-1252"},"PeriodicalIF":1.7000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Eye Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/02713683.2024.2380440","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/21 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"OPHTHALMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Purpose: To evaluate the protective role of Laurus Nobilis eye drops on selenite-induced cataracts in suckling rabbits.
Methods: Fifteen male albino suckling rabbits with no signs of ocular inflammation were randomly assigned to three groups: controls (Group A), sodium-selenite group (Group B) and sodium-selenite plus Laurus Nobilis group (Group C). By selenite treatment, cataract formation was experimentally induced and then graded. The grade of oxidative stress was defined in the lens, measuring the concentration of malondialdehyde, alpha-tocopherol, oxidized glutathione, ascorbic acid and hydrogen peroxide, and in blood samples as levels of alpha-tocopherol and malondialdehyde.
Results: Mean lens concentrations of GSSG, H2O2, and MDA levels in group B were significantly higher than in both group C and control. Ascorbic acid and alpha-tocopherol concentrations were lower in group B than in both group C and A. As plasma oxidative status markers, the level of MDA was higher in group B respected group C and A. The mean alpha-tocopherol levels in group B were significantly lower than in both group A and group C.
Conclusions: In animals treated with Laurus Nobilis-based eye drops, inflammation was inhibited, and lipid peroxidation was significantly reduced. Laurus nobilis leaves extract represents a good source of antioxidant components that may contrast sodium selenite-induced cataractogenesis in suckling rabbits.
目的：评估月桂叶滴眼液对亚硒酸钠诱发的乳兔白内障的保护作用：将15只无眼部炎症症状的雄性白化乳兔随机分为三组：对照组（A组）、亚硒酸钠组（B组）和亚硒酸钠加月桂叶滴眼液组（C组）。通过亚硒酸钠处理，实验诱导白内障形成，然后进行分级。在晶状体中测量丙二醛、α-生育酚、氧化谷胱甘肽、抗坏血酸和过氧化氢的浓度，在血液样本中测量α-生育酚和丙二醛的浓度，从而确定氧化应激的等级：B 组晶状体中 GSSG、H2O2 和 MDA 的平均浓度明显高于 C 组和对照组。抗坏血酸和α-生育酚浓度在 B 组低于 C 组和 A 组。作为血浆氧化状态标志物，MDA 水平在 B 组高于 C 组和 A 组：结论：使用月桂叶滴眼液治疗的动物，炎症得到了抑制，脂质过氧化反应明显降低。月桂叶提取物是一种很好的抗氧化成分来源，可抑制亚硒酸钠诱导的乳兔白内障发生。
期刊介绍：
The principal aim of Current Eye Research is to provide rapid publication of full papers, short communications and mini-reviews, all high quality. Current Eye Research publishes articles encompassing all the areas of eye research. Subject areas include the following: clinical research, anatomy, physiology, biophysics, biochemistry, pharmacology, developmental biology, microbiology and immunology.
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