In-vitro and in-vivo assessment of biological control potential of nematode symbiont Xenorhabdus nematophila against Pseudomonas syringae, the causative agent of angular leaf spot of cucumber
Muswar Ali Gadhi, Talha Nazir, Muhammad Zeeshan Majeed, Ghulam Hussain Jatoi, Ren Jie, Dewen Qiu
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引用次数: 0
Abstract
Bacterial diseases cause colossal losses in vegetable crops and are considered major limiting factors for the commercial production of vegetables around the globe. Among bacterial diseases, angular leaf spot of cucumber caused by Pseudomonas syringae is one of the most economically important diseases that cause substantial economic loss to the cucumber crop. In this study, the in-vitro and in-vivo effects of different strains (X1–X15) of the nematode symbiotic bacterium Xenorhabdus nematophila was determined on the growth of P. syringae in different selected media (TB, SB, SOB, SOC, YT and LB) and at different pH levels (6.5, 7.5 and 8.5) and on the incidence of angular leaf spot disease on potted cucumber plants. According to the results, maximum growth inhibition of P. syringe (25 mm) was observed in the case of strain X12, followed by strains X10 (23 mm), X8 (21 mm) and X6 (20 mm), while maximum colony growth was observed in the control. Moreover, this P. syringae growth inhibition by the X12 strain of X. nematophila was significantly maximum in SB medium and at pH 7.5. Results of the in-vivo experiment with potted plants showed a maximum incidence (90%) of angular leaf spot disease in control plants, while a minimum disease incidence (15%) was observed for the plants treated with two times dilution of X. nematophila culture, followed by ten times (23%) and fifty times (35%). The overall results of this study demonstrate the inhibitory effect of X. nematophila on the growth and incidence of P. syringae and underscore its putative role in the biological management of plant pathogenic bacteria.
期刊介绍:
Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays.
Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes.
Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.