In-vitro and in-vivo assessment of biological control potential of nematode symbiont Xenorhabdus nematophila against Pseudomonas syringae, the causative agent of angular leaf spot of cucumber

IF 1.1 4区 农林科学 Q3 PLANT SCIENCES
Muswar Ali Gadhi, Talha Nazir, Muhammad Zeeshan Majeed, Ghulam Hussain Jatoi, Ren Jie, Dewen Qiu
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Abstract

Bacterial diseases cause colossal losses in vegetable crops and are considered major limiting factors for the commercial production of vegetables around the globe. Among bacterial diseases, angular leaf spot of cucumber caused by Pseudomonas syringae is one of the most economically important diseases that cause substantial economic loss to the cucumber crop. In this study, the in-vitro and in-vivo effects of different strains (X1–X15) of the nematode symbiotic bacterium Xenorhabdus nematophila was determined on the growth of P. syringae in different selected media (TB, SB, SOB, SOC, YT and LB) and at different pH levels (6.5, 7.5 and 8.5) and on the incidence of angular leaf spot disease on potted cucumber plants. According to the results, maximum growth inhibition of P. syringe (25 mm) was observed in the case of strain X12, followed by strains X10 (23 mm), X8 (21 mm) and X6 (20 mm), while maximum colony growth was observed in the control. Moreover, this P. syringae growth inhibition by the X12 strain of X. nematophila was significantly maximum in SB medium and at pH 7.5. Results of the in-vivo experiment with potted plants showed a maximum incidence (90%) of angular leaf spot disease in control plants, while a minimum disease incidence (15%) was observed for the plants treated with two times dilution of X. nematophila culture, followed by ten times (23%) and fifty times (35%). The overall results of this study demonstrate the inhibitory effect of X. nematophila on the growth and incidence of P. syringae and underscore its putative role in the biological management of plant pathogenic bacteria.

线虫共生体 Xenorhabdus nematophila 对黄瓜角斑叶病病原菌 Pseudomonas syringae 的生物防治潜力的体外和体内评估
细菌性病害给蔬菜作物造成巨大损失,被认为是全球蔬菜商业化生产的主要限制因素。在细菌性病害中,由黄瓜假单胞菌(Pseudomonas syringae)引起的黄瓜角斑叶病是最具经济价值的病害之一,给黄瓜作物造成了巨大的经济损失。本研究测定了线虫共生菌 Xenorhabdus nematophila 的不同菌株(X1-X15)在不同选择培养基(TB、SB、SOB、SOC、YT 和 LB)和不同 pH 值(6.5、7.5 和 8.5)下对黄瓜假单胞菌生长的体外和体内影响,以及对盆栽黄瓜角斑叶病发病率的影响。结果表明,菌株 X12 对黄瓜角斑病的生长抑制最大(25 毫米),其次是菌株 X10(23 毫米)、X8(21 毫米)和 X6(20 毫米),而对照组的菌落生长最大。此外,X. nematophila 的 X12 菌株对 P. syringae 生长的抑制作用在 SB 培养基和 pH 值为 7.5 时明显最大。盆栽植物的体内实验结果表明,对照植物的角斑叶病发病率最高(90%),而用 2 倍稀释的 X. 线虫培养物处理的植物发病率最低(15%),其次是 10 倍(23%)和 50 倍(35%)。这项研究的总体结果证明了 X. 线虫对 P. syringae 的生长和发病率有抑制作用,并强调了它在植物病原菌生物管理中的潜在作用。
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来源期刊
Journal of Phytopathology
Journal of Phytopathology 生物-植物科学
CiteScore
2.90
自引率
0.00%
发文量
88
审稿时长
4-8 weeks
期刊介绍: Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.
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