Immunohistochemical analysis and distribution of epithelial mast cells in the rat larynx and trachea.

IF 2.1 4区 生物学 Q4 CELL BIOLOGY
Histochemistry and Cell Biology Pub Date : 2024-10-01 Epub Date: 2024-07-20 DOI:10.1007/s00418-024-02309-6
Sayed Sharif Abdali, Takuya Yokoyama, Yoshio Yamamoto, Keishi Narita, Masato Hirakawa, Tomoyuki Saino
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Abstract

Mast cells (MCs) in rat airways have been classified into two subtypes: epithelial MCs and connective tissue MCs (CTMCs). However, the immunohistochemical characteristics, cellular morphology, and distribution of epithelial MCs in the upper airways remain unclear. The present study investigated the morphological characteristics and distribution of epithelial MCs using 5-hydroxytryptamine (5-HT) and other immunohistochemical markers in sectioned or whole-mount preparations of the rat larynx and trachea. A double immunofluorescence analysis revealed the colocalization of 5-HT immunoreactivity with c-kit, a stem cell factor receptor commonly used as a MC marker, in both epithelial MCs and CTMCs. Dopa decarboxylase, an enzyme involved in 5-HT synthesis, was detected in both subtypes, suggesting their ability to synthesize and release 5-HT. Tryptase and histidine decarboxylase (a biosynthetic enzyme of histamine), which are well-known mediators of MCs, were exclusive to CTMCs. Epithelial MCs were pleomorphic with long cytoplasmic processes, whereas CTMCs were round and lacked cytoplasmic processes. The density of epithelial MCs was significantly higher in the glottis and cranial part of the trachea than in the epiglottis and other parts of the trachea. The present results showed that the morphology and immunohistochemical characteristics of epithelial MCs were different from those of CTMCs in the rat larynx and trachea, and variform epithelial MCs were predominantly located at the entrance of the upper airways. Epithelial MCs may release 5-HT to regulate innate immune responses by modulating epithelial cell functions at the entrance gate of the upper airways.

Abstract Image

大鼠喉和气管上皮肥大细胞的免疫组化分析和分布。
大鼠气道中的肥大细胞(MCs)被分为两种亚型:上皮 MCs 和结缔组织 MCs(CTMCs)。然而,上皮 MCs 在上呼吸道中的免疫组化特征、细胞形态和分布仍不清楚。本研究使用 5- 羟色胺(5-HT)和其他免疫组化标记物研究了大鼠喉和气管切片或整张制备的上皮 MCs 的形态特征和分布。双重免疫荧光分析显示,在上皮 MCs 和 CTMCs 中,5-HT 免疫反应与 c-kit 共定位,c-kit 是干细胞因子受体,常用作 MC 标记。在两种亚型中都检测到了多巴脱羧酶,这是一种参与 5-HT 合成的酶,表明它们具有合成和释放 5-HT 的能力。胰蛋白酶和组氨酸脱羧酶(组胺的一种生物合成酶)是众所周知的 MCs 介质,但 CTMCs 却独有这两种酶。上皮 MCs 呈多形性,胞质突起较长,而 CTMCs 则呈圆形,无胞质突起。声门和气管颅内上皮 MCs 的密度明显高于会厌和气管的其他部位。本研究结果表明,大鼠喉和气管上皮 MCs 的形态和免疫组化特征与 CTMCs 不同,且变异上皮 MCs 主要位于上呼吸道入口处。上皮 MCs 可能会释放 5-HT 来调节上呼吸道入口处上皮细胞的功能,从而调节先天性免疫反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Histochemistry and Cell Biology
Histochemistry and Cell Biology 生物-细胞生物学
CiteScore
4.90
自引率
8.70%
发文量
112
审稿时长
1 months
期刊介绍: Histochemistry and Cell Biology is devoted to the field of molecular histology and cell biology, publishing original articles dealing with the localization and identification of molecular components, metabolic activities and cell biological aspects of cells and tissues. Coverage extends to the development, application, and/or evaluation of methods and probes that can be used in the entire area of histochemistry and cell biology.
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