{"title":"Postharvest nicotinamide treatment modifies the redox status in highbush blueberry (Vaccinum corymbosum L.) fruit during storage","authors":"","doi":"10.1016/j.postharvbio.2024.113105","DOIUrl":null,"url":null,"abstract":"<div><p>Increased expression and activity of PARP family enzymes is associated with perturbations in the energy metabolism and redox status of the fruit after harvest. Because nicotinamide is a competitive inhibitor of PARP enzymes, in the present study we set out to check whether nicotinamide treatment of highbush blueberry fruit would affect PARP-1 expression, energy metabolism, and redox status in the fruit, and whether nicotinamide would protect the tissue from the pro-oxidative effects of UV-C radiation. For this purpose, the fruits of <em>Vaccinum corymbosum</em> L. cv. Duke, were treated with a 10 mM aqueous solution of nicotinamide and additionally co-treated with UV-C radiation, which were then stored for 7 d at 10 °C. We observed in the course of the study that nicotinamide clearly contributes to the reduction of oxidative stress in blueberry fruit. This was observed in the form of reduced PARP-1 expression in both UV-C irradiated and non-UV-C irradiated fruit. Reduced PARP-1 expression, in turn, inhibited NAD<sup>+</sup> loss, which contributed to higher ATP availability and consequently less ROS accumulation in the tissue. Furthermore, nicotinamide treatment reduced the decline of low-molecular weight of antioxidants content in UV-C-irradiated fruit.</p></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":null,"pages":null},"PeriodicalIF":6.4000,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Postharvest Biology and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0925521424003508","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
Increased expression and activity of PARP family enzymes is associated with perturbations in the energy metabolism and redox status of the fruit after harvest. Because nicotinamide is a competitive inhibitor of PARP enzymes, in the present study we set out to check whether nicotinamide treatment of highbush blueberry fruit would affect PARP-1 expression, energy metabolism, and redox status in the fruit, and whether nicotinamide would protect the tissue from the pro-oxidative effects of UV-C radiation. For this purpose, the fruits of Vaccinum corymbosum L. cv. Duke, were treated with a 10 mM aqueous solution of nicotinamide and additionally co-treated with UV-C radiation, which were then stored for 7 d at 10 °C. We observed in the course of the study that nicotinamide clearly contributes to the reduction of oxidative stress in blueberry fruit. This was observed in the form of reduced PARP-1 expression in both UV-C irradiated and non-UV-C irradiated fruit. Reduced PARP-1 expression, in turn, inhibited NAD+ loss, which contributed to higher ATP availability and consequently less ROS accumulation in the tissue. Furthermore, nicotinamide treatment reduced the decline of low-molecular weight of antioxidants content in UV-C-irradiated fruit.
期刊介绍:
The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages.
Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing.
Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.