Targeted analysis of organic acids with GC-MS/MS: Challenges and prospects

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2024-07-18 DOI:10.1016/j.ab.2024.115620

Jeremie Zander Lindeque

引用次数: 0

Abstract

GC-MS/MS combines the superior chromatographic resolution of GC with the specific and sensitive detection of tandem MS. On paper, it is an ideal system for the routine analyses of organic acids, yet very few studies have used and published such methods. This is likely due to several challenges highlighted in this communication. Briefly, the combination of EI ionization with MRM detection provides arguably insufficient specificity when targeting organic acids. Moreover, the narrow peaks generally produced by GC can lead to inaccurate quantification when the mass spectrometer's cycle time is too long. Potential solutions to these problems are discussed.

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利用 GC-MS/MS 对有机酸进行目标分析：挑战与前景。

GC-MS/MS 结合了气相色谱卓越的色谱分辨率和串联质谱特异灵敏的检测能力。从纸面上看，这是一种理想的有机酸常规分析系统，但使用和发表这种方法的研究却寥寥无几。这可能是由于本报告中强调的几个挑战造成的。简而言之，EI 离子化与 MRM 检测的结合在针对有机酸时提供的特异性不足。此外，当质谱仪的周期时间过长时，气相色谱通常产生的窄峰可能会导致定量不准确。本文讨论了这些问题的潜在解决方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.