Se-functionalized ZIF-8 nanoparticles: synthesis, characterization and disruption of biofilms and quorum sensing inSerratia marcescens.

Kunal M Gokhale, Vandana Patravale, Rutuja Pingale, Pooja Pandey, Sirisha L Vavilala
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Abstract

The majority of research on nanomaterials has been concentrated on metal nanoparticles since they are easily made and manipulated. Nanomaterials have shown a wide range of applications in biology. Nevertheless, their bioactivity declines due to their extreme susceptibility to and novel Se@ZIF-8 by chemical method. The sizes and morphologies of Se (0) and Se@ZIFchemical and physical stimuli. The goal of encapsulating these nanomaterials in a matrix is gradually being pursued, which boosts their affordability, stability, and usability. Metal-organic frameworks, often known as MOFs, have the potential to be the best platforms for encapsulating metal nanoparticles due to their well-defined frameworks, persistent porosity, and flexibility in modification. In this investigation, we report the synthesis and optimization of polyvinylpyrrolidone-stabilized Se(0) nanoparticles -8 were affected by the ratios of Se/Zn2+and [hmim]/Zn2+used. The optimized Se@ZIF-8 nanoparticles exhibited a particle size and zeta potential of 319 nm and -34 mv respectively. Transmission electron microscopy displayed spherical morphology for Se(0) nanoparticles, whereas the surface morphology of novel Se@ZIF-8 nanoparticles was drastically changed to hexagonal shaped structures with smooth surface morphologies in scanning electron microscopy (SEM). The DTA, TG/DTG, XRD analysis confirmed the presence of novel Se incorporated ZIF-8 nanoparticulate framework. The synthesized novel Se@ZIF-8 nanoparticles showed efficient antibacterial activity as evidenced by low MIC values. Interestingly, these Se@ZIF-8 NPs not only inhibited biofilm formation inS. marcescens,but also effectively eradicated mature biofilms by degrading the eDNA of the EPS layer. It was validated by confocal laser scanning microscopy and SEM analysis. It was observed that Se@ZIF-8 targeted the Quroum Sensing pathway and reduced its associated virulence factors production. This work opens up a different approach of Se@ZIF-8 nanoparticles as novel antibiotics to treat biofilm-associated infections caused byS. marcescensand offer a solution for antimicrobial resistance.

硒功能化 ZIF-8 纳米粒子:合成、表征和破坏 Serratia marcescens 的生物膜及法定量感应。
有关纳米材料的研究大多集中在金属纳米颗粒上,因为它们易于制造和操作。纳米材料在生物学中有着广泛的应用。然而,由于纳米材料极易受到化学和物理刺激的影响,其生物活性有所下降。将这些纳米材料封装在基质中的目标正在逐步实现,从而提高其经济性、稳定性和可用性。金属有机框架(通常称为 MOFs)具有框架明确、孔隙率持久、改性灵活等特点,有望成为封装金属纳米颗粒的最佳平台。本研究报告采用化学方法合成并优化了聚乙烯吡咯烷酮(PVP)稳定的 Se (0) 纳米粒子和新型 Se@ZIF-8。Se (0) 和 Se@ZIF-8 的尺寸和形态受所用 Se/Zn2+ 和 [hmim]/Zn2+ 比例的影响。优化后的 Se@ZIF-8 纳米粒子的粒径和 zeta 电位分别为 319 nm 和 -34 mv。透射电子显微镜下,Se(0) 纳米粒子呈球形,而扫描电子显微镜下,新型 Se@ZIF-8 纳米粒子的表面形态急剧变化为六边形结构,表面形态光滑。通过 DTA、TG/DTG 和 XRD 分析,证实了新型掺硒 ZIF-8 纳米颗粒框架的存在。合成的新型 Se@ZIF-8 纳米粒子具有高效的抗菌活性,其 MIC 值较低。有趣的是,这些 Se@ZIF-8 NPs 不仅能抑制 S. marcescens 的生物膜形成,还能通过降解 EPS 层的 eDNA 有效清除成熟的生物膜。据观察,Se@ZIF-8 针对的是 Quroum Sensing 通路,并减少了其相关毒力因子的产生。这项研究开辟了一种不同的方法,将Se@ZIF-8纳米粒子作为新型抗生素来治疗由S. marcescens引起的生物膜相关感染,并为抗菌药耐药性问题提供了一种解决方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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