Glutathione Levels and Lipid Oxidative Damage in Selected Organs of Obese Koletsky and Lean Spontaneously Hypertensive Rats.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-17 DOI:10.33549/physiolres.935319

H Rauchová, B Neprašová, L Maletínská, J Kuneš

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Abstract

Koletsky rats, the genetically obese strain of spontaneously hypertensive rats (SHROB), are the well-accepted animal model of human metabolic syndrome. They are characterized by early onset obesity, spontaneous hypertension, hyperinsulinemia, hyperlipidemia, proteinuria and shortened life-span. One of the factors in the pathogenesis of metabolic syndrome is oxidative stress. The aim of the present study was to compare two parameters related to oxidative stress: the levels of the main intracellular antioxidant, reduced glutathione as well as the indirect indicator of lipid peroxidation damage, thiobarbituric acid-reactive substances (TBARS) in heart, renal cortex and medulla and liver in male lean spontaneously hypertensive rats (SHR) and obese Koletsky rats. We did not find any significant differences in these markers in heart and kidneys. However, we found significantly lower glutathione level in Koletsky rat liver compared with SHR (5.03+/-0.23 vs. 5.83+/-0.14 µmol/g tissue, respectively). On the contrary, we observed significantly higher TBARS levels in Koletsky rat liver compared with SHR (28.56+/-2.15 vs. 21.83+/-1.60 nmol/mg protein, respectively). We conclude that the liver is the most sensitive tissue to oxidative damage with the significantly decreased concentration of glutathione and the significantly increased concentration of TBARS in obese Koletsky rats in comparison with lean control SHR.

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肥胖科莱茨基大鼠和瘦自发性高血压大鼠部分器官中的谷胱甘肽水平和脂质氧化损伤

科莱茨基大鼠是自发性高血压大鼠（SHROB）的遗传性肥胖品系，是公认的人类代谢综合征动物模型。它们的特点是早发性肥胖、自发性高血压、高胰岛素血症、高脂血症、蛋白尿和寿命缩短。氧化应激是代谢综合征的发病因素之一。本研究的目的是比较与氧化应激有关的两个参数：细胞内主要抗氧化剂还原型谷胱甘肽的水平，以及脂质过氧化损伤的间接指标硫代巴比妥酸反应物质（TBARS）在雄性自发性高血压瘦大鼠（SHR）和肥胖科莱茨基大鼠心脏、肾皮质和髓质以及肝脏中的水平。我们没有发现心脏和肾脏中的这些标记物有任何明显差异。然而，我们发现 Koletsky 大鼠肝脏中的谷胱甘肽水平明显低于 SHR（分别为 5.03+/-0.23 与 5.83+/-0.14 µmol/g 组织）。相反，我们观察到 Koletsky 大鼠肝脏中的 TBARS 水平明显高于 SHR（分别为 28.56+/-2.15 vs. 21.83+/-1.60 nmol/mg 蛋白质）。我们的结论是，与瘦对照组 SHR 相比，肥胖 Koletsky 大鼠肝脏中谷胱甘肽浓度明显降低，TBARS 浓度明显升高，因此肝脏是对氧化损伤最敏感的组织。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.