A CRISPR-amplified label-free electrochemical aptasensor for the sensitive detection of HbA1c†

IF 3.5 Q2 CHEMISTRY, ANALYTICAL
Jianfeng Ma, Youwei Zheng, Yaoyao Xie, Dan Zhu, Lianhui Wang and Shao Su
{"title":"A CRISPR-amplified label-free electrochemical aptasensor for the sensitive detection of HbA1c†","authors":"Jianfeng Ma, Youwei Zheng, Yaoyao Xie, Dan Zhu, Lianhui Wang and Shao Su","doi":"10.1039/D4SD00193A","DOIUrl":null,"url":null,"abstract":"<p >Glycated hemoglobin (HbA1c) is a pivotal biomarker for the monitoring and early diagnosis of diabetes. The CRISPR-Cas system has fascinating application prospects in the next generation of biosensors due to its high specificity, efficiency, flexibility, and customization. Herein, a label-free electrochemical aptasensor was designed for the detection of HbA1c by combining the specific recognition ability of aptamers with the signal amplification effect of the CRISPR-Cas12a system. In the presence of HbA1c, the <em>cis</em>–<em>trans</em> cleavage ability of Cas12a protein was activated, causing the pre-formed probe DNA to be heavily cleaved and the electrochemical signal to increase. With CRISPR-assisted signal amplification, the developed electrochemical aptasensor can detect as low as 0.84 ng mL<small><sup>−1</sup></small> HbA1c. Moreover, this aptasensor can detect 10 ng mL<small><sup>−1</sup></small> HbA1c in 50% human serum due to its high selectivity, reproducibility, and long-term stability, which is lower than its physiological level in human blood samples. All results proved that the proposed aptasensor has a promising application in the early diagnosis and long-term monitoring of diabetes.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 8","pages":" 1247-1252"},"PeriodicalIF":3.5000,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00193a?page=search","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors & diagnostics","FirstCategoryId":"1085","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/sd/d4sd00193a","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Glycated hemoglobin (HbA1c) is a pivotal biomarker for the monitoring and early diagnosis of diabetes. The CRISPR-Cas system has fascinating application prospects in the next generation of biosensors due to its high specificity, efficiency, flexibility, and customization. Herein, a label-free electrochemical aptasensor was designed for the detection of HbA1c by combining the specific recognition ability of aptamers with the signal amplification effect of the CRISPR-Cas12a system. In the presence of HbA1c, the cistrans cleavage ability of Cas12a protein was activated, causing the pre-formed probe DNA to be heavily cleaved and the electrochemical signal to increase. With CRISPR-assisted signal amplification, the developed electrochemical aptasensor can detect as low as 0.84 ng mL−1 HbA1c. Moreover, this aptasensor can detect 10 ng mL−1 HbA1c in 50% human serum due to its high selectivity, reproducibility, and long-term stability, which is lower than its physiological level in human blood samples. All results proved that the proposed aptasensor has a promising application in the early diagnosis and long-term monitoring of diabetes.

Abstract Image

用于灵敏检测 HbA1c 的 CRISPR 扩增无标记电化学适配传感器
糖化血红蛋白(HbA1c)是监测和早期诊断糖尿病的重要生物标志物。CRISPR-Cas 系统具有高特异性、高效性、灵活性和定制性等特点,在下一代生物传感器中具有广阔的应用前景。本文设计了一种无标记的电化学适配体传感器,将适配体的特异性识别能力与CRISPR-Cas12a系统的信号放大效应相结合,用于检测HbA1c。在 HbA1c 存在的情况下,Cas12a 蛋白的顺反裂解能力被激活,导致预先形成的探针 DNA 被大量裂解,电化学信号增加。通过 CRISPR 辅助信号放大,所开发的电化学适配传感器可以检测到低至 0.84 纳克/毫升的 HbA1c。此外,由于该传感器具有高选择性、可重复性和长期稳定性,它还能检测 50%人体血清中 10 ng/mL HbA1c 的含量,低于人体血样中的生理水平。所有结果都证明,该传感器在糖尿病的早期诊断和长期监测方面具有广阔的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
2.30
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信