{"title":"Development of Immunoradiometric Assay Technique for Measurement of Luteinizing Hormone in Human Serum","authors":"N. H. Ebeid, H. M. Shafik","doi":"10.1134/S1066362224030135","DOIUrl":null,"url":null,"abstract":"<p>This study was aimed to prepare immunoradiometric (IRMA) system using solid-phase magnetizable cellulose particles coupled with luteinizing hormone (LH) to determine LH in human serum. The LH-IRMA system was designed through preparation of LH polyclonal antibody (anti-LH), <sup>125</sup>I-LH tracer, and LH standards. Production of anti-LH was carried out through immunization of three Balb/C mice with LH-BSA immunogen. Magnetizable cellulose particles were activated using 1,1’-carbonyldiimidazole (CDI) and coupled with purified anti-LH. Optimization and validation of LH-IRMA were undertaken. The results obtained show that the local solid-phase magnetic particle LH-IRMA is a sensitive, specific, precise, and accurate method for measuring the LH concentration in human serum and can be used as a diagnostic tool for some disorders such as infertility in females and impotence in males.</p>","PeriodicalId":747,"journal":{"name":"Radiochemistry","volume":"66 3","pages":"383 - 393"},"PeriodicalIF":0.9000,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Radiochemistry","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1134/S1066362224030135","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}
引用次数: 0
Abstract
This study was aimed to prepare immunoradiometric (IRMA) system using solid-phase magnetizable cellulose particles coupled with luteinizing hormone (LH) to determine LH in human serum. The LH-IRMA system was designed through preparation of LH polyclonal antibody (anti-LH), 125I-LH tracer, and LH standards. Production of anti-LH was carried out through immunization of three Balb/C mice with LH-BSA immunogen. Magnetizable cellulose particles were activated using 1,1’-carbonyldiimidazole (CDI) and coupled with purified anti-LH. Optimization and validation of LH-IRMA were undertaken. The results obtained show that the local solid-phase magnetic particle LH-IRMA is a sensitive, specific, precise, and accurate method for measuring the LH concentration in human serum and can be used as a diagnostic tool for some disorders such as infertility in females and impotence in males.
期刊介绍：
Radiochemistry is a journal that covers the theoretical and applied aspects of radiochemistry, including basic nuclear physical properties of radionuclides; chemistry of radioactive elements and their compounds; the occurrence and behavior of natural and artificial radionuclides in the environment; nuclear fuel cycle; radiochemical analysis methods and devices; production and isolation of radionuclides, synthesis of labeled compounds, new applications of radioactive tracers; radiochemical aspects of nuclear medicine; radiation chemistry and after-effects of nuclear transformations.
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