Development of an indirect ELISA for the immunoprotection evaluation of E2 antibodies against classical swine fever virus

IF 2.2 4区 医学 Q3 BIOCHEMICAL RESEARCH METHODS
Qi Fang , Ye Luo , Tongtong Liang , Rongli Liao , Xiaohang Yu , Jin Zheng , Deming Yin , Xinglong Yu
{"title":"Development of an indirect ELISA for the immunoprotection evaluation of E2 antibodies against classical swine fever virus","authors":"Qi Fang ,&nbsp;Ye Luo ,&nbsp;Tongtong Liang ,&nbsp;Rongli Liao ,&nbsp;Xiaohang Yu ,&nbsp;Jin Zheng ,&nbsp;Deming Yin ,&nbsp;Xinglong Yu","doi":"10.1016/j.jviromet.2024.114999","DOIUrl":null,"url":null,"abstract":"<div><p>The Chinese government's reclassification of Classical Swine Fever (CSF) from a class Ⅰ to a class Ⅱ animal infectious disease, now also including CSF under the disease eradication program, reflects the significant progress made through extensive immunization with CSF vaccines. In light of this advancement, there is an imperative need for an expedient and accurate method to assess the levels of immunoprotection against classical swine fever virus (CSFV) in vaccinated pigs, a critical component in the campaign to eradicate the disease. This study develops an indirect enzyme-linked immunosorbent assay (iELISA) based on a highly glycosylated E2 protein stable expressed in CHO-K1 mammalian cells. Statistical analysis revealed strong positive correlations between the iELISA and VNT results (<em>r</em> = 0.9063, <em>p</em> &lt; 0.0001) that were much greater than those between the IDEXX ELISA and VNT results (<em>r</em> = 0.8126, <em>p</em> &lt; 0.0001). Taking the VNT data as the standard, the consistency of the iELISA (<em>κ</em> =0.880) was greater than that of the IDEXX ELISA (<em>κ</em> =0.699). In summary, the iELISA provides a more efficient and precise method for assessing CSFV immunity in pigs. Its reliable detection of immunoprotection levels against CSFV makes it an essential tool for optimizing CSF vaccination strategies. Consequently, its application can significantly support the ongoing efforts to eradicate CSF.</p></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"329 ","pages":"Article 114999"},"PeriodicalIF":2.2000,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of virological methods","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S016609342400123X","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

The Chinese government's reclassification of Classical Swine Fever (CSF) from a class Ⅰ to a class Ⅱ animal infectious disease, now also including CSF under the disease eradication program, reflects the significant progress made through extensive immunization with CSF vaccines. In light of this advancement, there is an imperative need for an expedient and accurate method to assess the levels of immunoprotection against classical swine fever virus (CSFV) in vaccinated pigs, a critical component in the campaign to eradicate the disease. This study develops an indirect enzyme-linked immunosorbent assay (iELISA) based on a highly glycosylated E2 protein stable expressed in CHO-K1 mammalian cells. Statistical analysis revealed strong positive correlations between the iELISA and VNT results (r = 0.9063, p < 0.0001) that were much greater than those between the IDEXX ELISA and VNT results (r = 0.8126, p < 0.0001). Taking the VNT data as the standard, the consistency of the iELISA (κ =0.880) was greater than that of the IDEXX ELISA (κ =0.699). In summary, the iELISA provides a more efficient and precise method for assessing CSFV immunity in pigs. Its reliable detection of immunoprotection levels against CSFV makes it an essential tool for optimizing CSF vaccination strategies. Consequently, its application can significantly support the ongoing efforts to eradicate CSF.

开发一种间接酶联免疫吸附试验(ELISA),用于评估针对经典猪瘟病毒的 E2 抗体的免疫保护作用
中国政府将猪瘟(CSF)从Ⅰ级动物传染病调整为Ⅱ级动物传染病,并将猪瘟列入疫病根除计划,这反映了通过广泛免疫猪瘟疫苗所取得的重大进展。鉴于这一进展,亟需一种便捷而准确的方法来评估接种疫苗的猪对经典猪瘟病毒(CSFV)的免疫保护水平,这是根除该疾病的关键组成部分。本研究基于在 CHO-K1 哺乳动物细胞中稳定表达的高度糖基化 E2 蛋白,开发了一种间接酶联免疫吸附测定法(iELISA)。统计分析显示,iELISA 和 VNT 结果(r = 0.9063,p < 0.0001)之间存在很强的正相关性,远高于 IDEXX ELISA 和 VNT 结果(r = 0.8126,p < 0.0001)之间的相关性。以 VNT 数据为标准,iELISA 的一致性(κ =0.880)高于 IDEXX ELISA(κ =0.699)。总之,iELISA 为评估猪 CSFV 免疫力提供了一种更高效、更精确的方法。它对 CSFV 免疫保护水平的可靠检测使其成为优化 CSF 疫苗接种策略的重要工具。因此,iELISA 的应用可以极大地支持目前为根除 CSF 所做的努力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
5.80
自引率
0.00%
发文量
209
审稿时长
41 days
期刊介绍: The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信