Phage based biosensors: Enhancing early detection of emerging pathogens in diagnostics

IF 4.1 Q1 CHEMISTRY, ANALYTICAL
Pooja Sachdeva , Gopal Nath , Utkarsh Jain
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引用次数: 0

Abstract

Global health is seriously threatened by an increase in antibiotic resistance among ESKAPE pathogens- E- E. faecium, S- S.aureus, K- K.pneumoniae, A-A.baumannii, P- P.aeruginosa, and E-Enterobacter. The resistance of many bacteria to traditional antibiotics is increasing, making the search for novel approaches critical. In order to minimize the effect of these diseases, early detection, diagnosis, and treatment are important. However, there are drawbacks to traditional detection techniques such molecular-based, biochemical, and microbiological assays. These include the inability to detect on-site, as well as their time-consuming, expensive, and labour-intensive nature. Viral agents that target bacteria exclusively, known as bacteriophages, have shown promise in combating over infections resistant to antibiotics. Bacteriophage-based biosensors are adaptable to many environmental conditions and offer special features such as host specificity and ability to identify active infections. They're very accurate, very specific, and have quick assay times, which makes them beneficial tools for detection. Also, phages are more easily produced than antibodies and can withstand high pH, temperature, and chemical solvents. The potential of bacteriophage-based biosensors in the fight against ESKAPE pathogens is highlighted by this review. Bacteriophage-based biosensors provide simplified detection processes in contrast to conventional approaches, which makes them invaluable in environmental and clinical situations. Numerous platforms, including electrochemical, magnetoelastic, quartz crystal microbalance, and surface plasmon resonance sensors, being investigated for their potential use to detect pathogenic bacteria in a range of sample types.

Abstract Image

基于噬菌体的生物传感器:加强诊断中对新兴病原体的早期检测
ESKAPE病原体(E-粪肠球菌、S-金黄色葡萄球菌、K-肺炎双球菌、A-鲍曼尼杆菌、P-绿脓杆菌和E-肠杆菌)对抗生素的耐药性增加严重威胁着全球健康。许多细菌对传统抗生素的耐药性正在增加,因此寻找新方法变得至关重要。为了将这些疾病的影响降至最低,早期检测、诊断和治疗非常重要。然而,传统检测技术(如分子检测、生化检测和微生物检测)存在一些缺点。这些缺点包括无法现场检测,以及耗时、昂贵和劳动密集型。专门针对细菌的病毒制剂(即噬菌体)在抗击对抗生素产生抗药性的过度感染方面大有可为。基于噬菌体的生物传感器可适应多种环境条件,并具有宿主特异性和识别活动感染的能力等特殊功能。它们非常准确、特异,检测时间短,是检测的有利工具。此外,噬菌体比抗体更容易生产,能承受高 pH 值、高温和化学溶剂。本综述强调了基于噬菌体的生物传感器在抗击 ESKAPE 病原体方面的潜力。与传统方法相比,基于噬菌体的生物传感器简化了检测过程,这使其在环境和临床应用中具有重要价值。目前正在研究包括电化学、磁弹性、石英晶体微天平和表面等离子体共振传感器在内的多种平台,以确定它们是否可用于检测各种类型样品中的致病细菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Talanta Open
Talanta Open Chemistry-Analytical Chemistry
CiteScore
5.20
自引率
0.00%
发文量
86
审稿时长
49 days
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