Effectiveness of Hypertonic Saline, Polidocanol, and Glycerol as Sclerosing Agent: An Experimental Study in Javan Rabbits (Lepus nigricollis)

Harlan, Mulawardi Mulawardi, S. Laidding, Firdaus Hamid, M. H. Cangara, T. C. Adriani, M. I. Kusuma, Prihantono, Warsinggih, Muhammad Faruk
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Abstract

INTRODUCTION: Therapeutic approaches to varicose veins include sclerotherapy, laser ablation, and surgery. Using sclerosing agents such as hypertonic saline, polidocanol, and glycerol can be an option in cases of recurrent varicose veins. This study aimed to assess the effectiveness of hypertonic saline, polidocanol, and glycerol as sclerosant agents in an in vivo study. MATERIAL AND METHODS: This was an experimental study on 24 Javan rabbits, divided into three treatment groups: hypertonic saline (group I, n=8), polidocanol (group II, n=8), and glycerol (group III, n=8). All animals received treatment by injection into the vein behind the ear, then vein damming 10 minutes later. Punch tissue samples for standard histopathological examination were taken from blood vessels at 1 hour, 24 hours, 7 days, and 45 days post-treatment across all groups. The histopathology changes were scored based on inflammation, proliferation, luminal narrowing, and fibrosis. RESULT: No differences were observed in the degrees of inflammation, proliferation, luminal narrowing, or fibrosis at different observation intervals. However, a significant and positive correlation was found between inflammation, vascular proliferation, and fibrosis with all sclerosing agents (p<0.005). No significant correlation exists in the scoring of luminal narrowing among any sclerosing agent (p>0.005). CONCLUSION: Hypertonic saline, polidocanol, and glycerol demonstrated comparable efficacy as sclerosing agents in vivo concerning fibrosis, vascular proliferation, and inflammation.
高渗盐水、波利多卡诺和甘油作为硬化剂的效果:爪哇兔(Lepus nigricollis)的实验研究
简介:静脉曲张的治疗方法包括硬化疗法、激光消融术和手术。在静脉曲张反复发作的情况下,可以选择使用硬化剂,如高渗盐水、聚多康醇和甘油。本研究旨在通过一项体内研究评估高渗盐水、聚多康醇和甘油作为硬化剂的有效性。材料与方法:本研究以 24 只爪哇兔为实验对象,分为三个治疗组:高渗盐水组(I 组,8 只)、多效唑组(II 组,8 只)和甘油组(III 组,8 只)。所有动物均接受耳后静脉注射治疗,10 分钟后进行静脉阻断。各组动物分别在治疗后 1 小时、24 小时、7 天和 45 天从血管中提取冲孔组织样本进行标准组织病理学检查。根据炎症、增生、管腔狭窄和纤维化对组织病理学变化进行评分。结果:在不同的观察时间间隔内,炎症、增生、管腔狭窄或纤维化程度均无差异。然而,炎症、血管增生和纤维化与所有硬化剂之间均存在明显的正相关性(P0.005)。结论:高渗盐水、多聚甲醛和甘油作为体内硬化剂,在纤维化、血管增生和炎症方面的疗效相当。
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