{"title":"Investigation of Iris versicolor metabolic profile and optimization of the isolation of bioactive components on a semi-operation scale","authors":"","doi":"10.1016/j.procbio.2024.07.001","DOIUrl":null,"url":null,"abstract":"<div><p>In this study, we aimed to (i) better understand the phytochemical composition of extracts prepared from the leaves and roots with rhizomes of the scarcely studied <em>Iris versicolor</em>, and (ii) propose optimal extraction conditions (solvent composition, temperature, time) for the isolation of bioactive compounds. A UHPLC-QTOF-MS/MS targeted screening method was applied in combination with in silico fragmentation. The analysis revealed a total of 106 target metabolites, many of which are reported to occur in <em>I. versicolor</em> for the first time. (Iso)flavonoids were the most abundant compounds, followed by terpenoids, phenols, xanthones, phenolic acids, quinones, and steroids, with quinones being reported for the first time in this species. Terpenoids were characteristic of roots with rhizomes, while xanthones dominated in the leaves. Based on the varied experimental conditions, surface response analysis generated significant models for the extraction of terpenoids, quinones, and steroids. Our research provides valuable insight for the effective medicinal and commercial use of <em>I. versicolor.</em></p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Process Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1359511324002228","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
In this study, we aimed to (i) better understand the phytochemical composition of extracts prepared from the leaves and roots with rhizomes of the scarcely studied Iris versicolor, and (ii) propose optimal extraction conditions (solvent composition, temperature, time) for the isolation of bioactive compounds. A UHPLC-QTOF-MS/MS targeted screening method was applied in combination with in silico fragmentation. The analysis revealed a total of 106 target metabolites, many of which are reported to occur in I. versicolor for the first time. (Iso)flavonoids were the most abundant compounds, followed by terpenoids, phenols, xanthones, phenolic acids, quinones, and steroids, with quinones being reported for the first time in this species. Terpenoids were characteristic of roots with rhizomes, while xanthones dominated in the leaves. Based on the varied experimental conditions, surface response analysis generated significant models for the extraction of terpenoids, quinones, and steroids. Our research provides valuable insight for the effective medicinal and commercial use of I. versicolor.
期刊介绍:
Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.