Quantitative Trait Mapping for Zebra-stem in Tomato Confirms a Genetic Cause Involving the Interaction of Unlinked Loci

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-01 DOI:10.21273/hortsci17766-24

Su Subode, Jihuen Cho, D. M. Francis

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Abstract

Zebra-stem of tomato is a disorder characterized by leaf necrosis, wilting, and a stripped pattern on stems of mature plants. Wilting, necrosis, and death of seedlings are also observed. The physiological and genetic causes of zebra-stem are poorly characterized. Anecdotal evidence has suggested pedigrees with S. pimpinellifolium and bacterial speck resistance in the genetic background are often prone to this disorder. We demonstrate a genetic cause using composite interval mapping and association analysis approaches to define quantitative trait loci (QTLs) that contribute to the disorder. A biparental population of F4 partially inbred families was developed for the initial analysis, and four subsequent backcross or F2 populations were used for subsequent validation. Significant QTLs on chromosomes 5 and 10 were identified, explaining ∼60% and 40% of the variation, respectively. Polymorphisms in the Pto locus are strongly associated with the QTL on chromosome 5. The two loci were derived from different parentage, and a significant interaction effect was demonstrated, resulting in the characteristic zebra-stem symptoms when combined.

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番茄斑马茎的定量性状图谱证实了非连锁位点相互作用的遗传原因

番茄斑马茎病是一种以成熟植株的叶片坏死、枯萎和茎上出现条纹为特征的疾病。秧苗也会出现萎蔫、坏死和死亡。斑马茎病的生理和遗传原因尚不明确。轶事证据表明，具有 S. pimpinellifolium 和细菌斑点抗性遗传背景的血统通常容易出现这种病症。我们利用复合间隔图谱和关联分析方法确定了导致这种疾病的数量性状位点（QTLs），从而证明了遗传原因。我们建立了一个由 F4 部分近交家系组成的双亲群体进行初步分析，并使用四个回交或 F2 群体进行后续验证。在 5 号和 10 号染色体上发现了显著的 QTLs，分别解释了 60% 和 40% 的变异。Pto 基因座的多态性与 5 号染色体上的 QTL 密切相关。这两个基因座来源于不同的亲本，并表现出显著的交互效应，结合后产生了特征性的斑马茎症状。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.