Systemic Effects of Photoactivated 5,10,15,20-tetrakis(N-methylpyridinium-3-yl) Porphyrin on Healthy Drosophila melanogaster

IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BioTech Pub Date : 2024-07-03 DOI:10.3390/biotech13030023
Ana Filošević Vujnović, Sara Čabrijan, Martina Mušković, Nela Malatesti, Rozi Andretić Waldowski
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引用次数: 0

Abstract

Porphyrins are frequently employed in photodynamic therapy (PDT), a non-invasive technique primarily utilized to treat subcutaneous cancers, as photosensitizing agents (PAs). The development of a new PA with improved tissue selectivity and efficacy is crucial for expanding the application of PDT for the management of diverse cancers. We investigated the systemic effects of 5,10,15,20-tetrakis(N-methylpyridinium-3-yl)-porphyrin (TMPyP3) using Drosophila melanogaster adult males. We established the oral administration schedule and demonstrated that TMPyP3 was absorbed and stored higher in neuronal than in non-neuronal extracts. Twenty-four hours after oral TMPyP3 photoactivation, the quantity of hydrogen peroxide (H2O2) increased, but exclusively in the head extracts. Regardless of photoactivation, TMPyP3 resulted in a reduced concentration of H2O2 after 7 days, and this was linked with a decreased capacity to climb, as indicated by negative geotaxis. The findings imply that systemic TMPyP3 therapy may disrupt redox regulation, impairing cellular signaling and behavioral outcomes in the process. To determine the disruptive effect of porphyrins on redox homeostasis, its duration, and the mechanistic variations in retention across various tissues, more research is required.
光活化 5,10,15,20-四(N-甲基吡啶-3-基)卟啉对健康黑腹果蝇的系统影响
光动力疗法(PDT)是一种主要用于治疗皮下癌症的非侵入性技术,卟啉作为光敏剂(PAs)经常被用于该疗法中。开发一种具有更好的组织选择性和疗效的新型光敏剂对于扩大光动力疗法在治疗各种癌症中的应用至关重要。我们利用黑腹果蝇成年雄性研究了 5,10,15,20-四(N-甲基吡啶-3-基)-卟啉(TMPyP3)的全身效应。我们确定了口服给药时间表,并证明神经元提取物对 TMPyP3 的吸收和储存量高于非神经元提取物。口服 TMPyP3 光活化 24 小时后,过氧化氢(H2O2)的数量增加,但仅限于头部提取物。无论光激活与否,TMPyP3 都会在 7 天后导致 H2O2 浓度降低,这与爬行能力下降有关,负向地心引力就表明了这一点。研究结果表明,全身性 TMPyP3 治疗可能会破坏氧化还原调节,在此过程中损害细胞信号传导和行为结果。要确定卟啉对氧化还原平衡的破坏作用、持续时间以及在不同组织中保留的机理变化,还需要进行更多的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BioTech
BioTech Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
3.70
自引率
0.00%
发文量
51
审稿时长
11 weeks
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