Clonal micropropagation and ex-situ conservation of Rhycncholaelia digbyana (Lindley) Schltr

Agro Productividad Pub Date : 2024-07-03 DOI:10.32854/agrop.v17i6.2832

R. Chi-Ramírez Maura, G. J. Herrera-Cool, A. Sánchez-Contreras, Guadalupe Lopez-Puc

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Abstract

Objective: To obtain protocols for clonal micropropagation, crop planning, and in vitro conservation of Rhycholaelia digbyana (Lindley) Schltr. Methodology: The effects of the Kundson C basal medium and benzylaminopurine concentration were evaluated for clonal micropropagation. The treatment with the greatest number of shoots formed per apex was selected for crop planning. Experiments were conducted to determine the effect of basal medium Murashige and Skoog concentration at 2.2 gL-1 and 4.4 gL-1; sorbitol, mannitol, and sucrose at 1, 2, and 3% on slow growth. Results: The best treatment for clonal micropropagation and crop planning was identified as 21.60 gL-1 Knudson C with 8.80 µM benzylaminopurine. This treatment resulted in uniform-sized shoots produced. The multiplication process can yield 10,240 seedlings in 12 months. Slow growth was achieved using Murashige and Skoog basal media at 2.2 gL-1 with 1% mannitol. Implications: More experiments must be conducted to determine the best shoot induction conditions and improve resource efficiency. Conclusions: These findings represent the first report on micropropagation and ex-situ conservation to preserve germplasm for this species as an important resource for the floriculture industry.

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Rhycncholaelia digbyana (Lindley) Schltr 的克隆微繁殖和异地保护

目的获得 Rhycholaelia digbyana (Lindley) Schltr 的克隆微繁殖、作物规划和离体保存规程。方法：用 Kundson C 作基质：评估了 Kundson C 基础培养基和苄氨基嘌呤浓度对克隆微繁殖的影响。选择每个先端形成的芽数量最多的处理进行作物规划。实验确定了基础培养基 Murashige 和 Skoog 浓度为 2.2 gL-1 和 4.4 gL-1；山梨醇、甘露醇和蔗糖浓度为 1%、2% 和 3%对缓慢生长的影响。结果克隆微繁殖和作物规划的最佳处理方法是 21.60 gL-1 Knudson C 和 8.80 µM 苄基氨基嘌呤。这种处理方法产生的芽大小一致。繁殖过程可在 12 个月内培育出 10 240 株幼苗。使用 2.2 gL-1 含有 1%甘露醇的 Murashige 和 Skoog 基础培养基可实现缓慢生长。影响：必须进行更多的实验，以确定最佳的芽诱导条件，提高资源效率。结论：这些研究结果是关于微繁殖和异地保护的首次报告，目的是保存该物种的种质资源，使其成为花卉栽培行业的重要资源。

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Agro Productividad

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