Physiological, genetical changes and cdc2 gene expression for osmotic stressed Vicia faba reveal the alleviation effect of gamma radiation and putrescine

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences

Caryologia Pub Date : 2024-07-08 DOI:10.36253/caryologia-2338

Shaimaa S. Sobieh, Noha Eid Eliwa

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引用次数: 0

Abstract

Climate change caused increasing in soil salinity worldwide. Therefore, it is critical to enhance the capability of plant to tolerate salinity stress. For this goal, putrescine and irradiation by gamma radiation were used to improve the salt tolerance of Vicia faba (the most important human crop). The results indicated depression in mitotic division and all growth parameters associated with the induction of micronucleus (MN) when salinity increased to 100mM, while there was increase in mitotic aberrations. NaCl decreased total soluble sugar, while total N%, total free amino acid, proline and protein contents showed slight increase with increasing salinity stress. Putrescine and gamma radiation mitigated the effect of salinity on cell division and growth parameters. Salt stress decreased the expression of gene encoding cyclin-dependent kinase 2 (cdc2). Both putrescine and gamma radiation increased cdc2 expression. The genetic diversity has been detected among control and treated V. faba using ISSR and SCoT markers. Ten primers had successfully generated 129 reproducible polymorphic amplicons that were suitable for studying the genetic diversity between studied genotypes. ISSR markers provided more discriminating data and were more informative than SCoT markers. Besides, cluster analysis using UPGMA and PAC successfully explained the genetic diversity within studied genotypes. These findings emphasize the efficiency of putrescine and gamma radiation for alleviating the negative impact of salt stress. Moreover, prove the importance of assessing mitotic activity, chromosomes behavior, physiological parameters, the expression level of cdc2 and molecular diversity in V. faba under stress to improve the salt tolerance of it.

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渗透压胁迫下紫花苜蓿的生理、基因变化和 cdc2 基因表达揭示了伽马射线和腐胺的缓解作用

气候变化导致全球土壤盐度不断增加。因此，提高植物耐盐碱胁迫的能力至关重要。为此，研究人员利用腐胺和伽马射线照射来提高紫花苜蓿（人类最重要的农作物）的耐盐性。结果表明，当盐度升高到 100 毫摩尔时，有丝分裂和与诱导微核（MN）相关的所有生长参数都受到抑制，同时有丝分裂畸变增加。随着盐度胁迫的增加，氯化钠降低了总可溶性糖含量，而总氮、总游离氨基酸、脯氨酸和蛋白质含量则略有增加。普氏素和γ射线减轻了盐胁迫对细胞分裂和生长参数的影响。盐胁迫降低了细胞周期蛋白依赖性激酶 2（cdc2）基因的表达。腐胺和伽马射线都增加了 cdc2 的表达。利用 ISSR 和 SCoT 标记检测了对照组和处理组蚕豆的遗传多样性。十种引物成功地产生了 129 个可重复的多态性扩增子，这些扩增子适用于研究基因型之间的遗传多样性。与 SCoT 标记相比，ISSR 标记提供的数据更具区分性，信息量更大。此外，使用 UPGMA 和 PAC 进行聚类分析，成功地解释了所研究基因型内部的遗传多样性。这些研究结果表明，腐胺和伽马射线能有效缓解盐胁迫的负面影响。此外，还证明了评估胁迫下蚕豆的有丝分裂活动、染色体行为、生理参数、cdc2 的表达水平和分子多样性对提高其耐盐性的重要性。

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.

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