Potential of Pandan anggur (Sararanga Sinuosa Hemsley) as immunomodulator

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-08 DOI:10.31989/ffhd.v14i7.1378

Lefaan Paula, Nancy, Murdiati Agnes, Fibri Dwi, Larasatie Nur, Prof Nurliyani

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引用次数: 0

Abstract

Background: Pandan Anggur plant is an endemic plant of the Papua archipelago in Indonesia, and the fruit called Pandan Anggur Fruit (PAF) is freshly consumed or prepared as a juice. Based on previous studies on the phytochemical content of PAF, the ethanol and water extract contain flavonoids, which act as immunomodulators, to regulate the response of immunity. Objectives: This study aimed to examine the potential of PAF as an immunomodulator in malnutrition cases, by evaluating six parameters which are phagocytosis capacity and nitric oxide (NO) production of peritoneal macrophage, spleen lymphocyte proliferation, IFN-γ and IL-4 in lymphocyte culture, and intestinal sIgA levels. By proving the potential of PAF as an immunomodulator, this research will lead to significant development of functional food products of Pandan Anggur. Methods: A total of 35 Sprague Dawley rats were used as animal models divided into seven different groups named standard group (healthy standard), malnourished (negative control), malnourished rats given Imboost Force (positive control), as well as PAF ethanol extract doses of I, II, III, and PAF water extract. The treatment lasted for seven weeks in total and the measurement of immunomodulator parameters was carried out at the end of the treatment period. The immunomodulator parameters included phagocytosis capacity and nitric oxide (NO) production of peritoneal macrophage, spleen lymphocyte proliferation, IFN-γ and IL-4 in lymphocyte culture, and intestinal sIgA levels. Result: The results indicated that the administration of PAF ethanol extract at a dose of 3.15 mg/mL provided an optimum immunostimulant effect, by increasing the macrophage phagocytosis capacity, nitric oxide, lymphocyte proliferation, IL-4, and sIgA levels in rat intestine. However, increasing the dose of extract did not produce a better effect, but led to suppression of immunity. Conclusion: PAF extract at a dose of 3.15 mg/mL provides an immunostimulant effect. Keywords: In Vivo, IL-4, IF-γ, sIgA, lymphocyte proliferation, macrophage, Pandan Anggur Fruit

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露兜树（Sararanga Sinuosa Hemsley）作为免疫调节剂的潜力

背景介绍Pandan Anggur 植物是印度尼西亚巴布亚群岛的特有植物，其果实被称为 Pandan Anggur 果（PAF），可新鲜食用或榨汁。根据以往对 PAF 植物化学成分的研究，乙醇和水提取物中含有黄酮类化合物，可作为免疫调节剂调节免疫反应。研究目的本研究旨在通过评估腹腔巨噬细胞的吞噬能力和一氧化氮（NO）产生、脾脏淋巴细胞增殖、淋巴细胞培养中的 IFN-γ 和 IL-4 以及肠道 sIgA 水平等六个参数，研究 PAF 作为免疫调节剂在营养不良病例中的潜力。通过证明 PAF 作为一种免疫调节剂的潜力，这项研究将为开发潘丹鲡功能性食品带来重大意义。研究方法以 35 只 Sprague Dawley 大鼠为动物模型，分为 7 个不同的组别，分别为标准组（健康标准）、营养不良组（阴性对照）、给予 Imboost Force（阳性对照）和 PAF 乙醇提取物剂量 I、II、III 及 PAF 水提取物的营养不良组。治疗共持续七周，在治疗期结束时测量免疫调节剂参数。免疫调节指标包括腹腔巨噬细胞的吞噬能力和一氧化氮（NO）产生、脾脏淋巴细胞增殖、淋巴细胞培养中的IFN-γ和IL-4以及肠道sIgA水平。结果结果表明，服用 3.15 mg/mL 剂量的 PAF 乙醇提取物可提高大鼠肠道巨噬细胞吞噬能力、一氧化氮、淋巴细胞增殖、IL-4 和 sIgA 水平，从而产生最佳的免疫刺激效果。然而，增加提取物的剂量并不能产生更好的效果，反而会导致免疫抑制。结论3.15毫克/毫升剂量的PAF提取物具有免疫刺激作用。关键词体内 IL-4、IF-γ、sIgA、淋巴细胞增殖、巨噬细胞、潘丹安格斯果

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.