Exploring the interplay between host plant structural defences and origin on behavioural and life-history traits of two Neotropical danaines

Abstract

Factors that mediate insect herbivore sabotaging behaviour of host plant latex defences remain under debate. The most prominent hypothesis suggests that the anatomy of the host plant's laticifers determines trenching or vein-cutting behaviour, but there are inconsistencies in the literature. In addition to latex, other plant defences have been shown to affect plant natural enemies, but experimental studies comparing the effects of multiple defences on herbivore performance and behaviour are scarce. In this study, we investigated the anatomy of the laticifers of Calotropis procera (Aiton) W.T. Aiton (Apocynaceae), an exotic milkweed of southeastern Brazil, as well as the sabotaging behaviour of larvae of southern monarch, Danaus erippus (Cramer), and queen butterfly, Danaus gilippus (Cramer) (Lepidoptera: Nymphalidae) on this host plant. We also reviewed the sabotaging behaviour of larvae reared on the native milkweed Asclepias curassavica L. (Apocynaceae) from previous studies, as well as the description of the anatomy of its laticifers. As we found no correspondence between sabotaging behaviour of southern monarchs and queens and the anatomy of the host plants' laticifers, we tested whether structural defences of C. procera leaves (latex, waxes and trichomes) affected sabotaging behaviour and larval performance of southern monarch larvae. The structural barriers of this well-defended host affected larvae in different ways: Southern monarchs developed more slowly on control leaves than on leaves from which wax had been removed, and only latex removal reduced the frequency of sabotaging behaviour. Regardless of the defence removal treatment, frequencies of trenching behaviour decreased and vein-cutting behaviour increased as larvae developed. Overall, our results show that several factors in addition to the anatomy of laticifers affect sabotaging behaviour of southern monarchs and queens, including larval ontogeny, danaine species and latex outflow. This evidence suggests a behavioural plasticity in the sabotaging behaviour of larvae of both southern monarchs and queens.