Development of stability indicating RP-HPLC method for Quercetin estimation in Nanostructure liquid crystalline dispersion

IF 0.1 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

International Journal of Ayurvedic Medicine Pub Date : 2024-07-10 DOI:10.47552/ijam.v15i2.4421

Bhaskar K. Kurangi, Sunil S. Jalalpure, Mayuri Joshi, Umesh Patil

{"title":"Development of stability indicating RP-HPLC method for Quercetin estimation in Nanostructure liquid crystalline dispersion","authors":"Bhaskar K. Kurangi, Sunil S. Jalalpure, Mayuri Joshi, Umesh Patil","doi":"10.47552/ijam.v15i2.4421","DOIUrl":null,"url":null,"abstract":"An attempt has been made to develop stability representing the reverse-phase High-Performance Liquid Chromatography (RP-HPLC) method for quantitative estimation of quercetin Nanostructure liquid crystalline dispersion. HPLC method was developed by C-18 column with the mobile phase comprising of 0.4% orthophosphoric acid [OPA]: Acetonitrile [ACN] (45:55, v/v), isocratically pumped with a rate of flow 1 mL/min and 255 nm detection wavelength. The method validation of HPLC parameters and degradation tests was analysed by International Conference on Harmonization (ICH) guidelines. The developed HPLC method was found to be specific, linear (r2>0.999) over the selected range of concentration 2-14μg/mL, precise, with the limit of detection (LOD) and limit of quantification (LOQ) as 0.021 and 0.064μg/mL respectively. The entrapment efficiency of quercetin for prepared Nanostructure Liquid Crystalline Dispersion (NLCD) was observed to be 94.55 %. The developed HPLC method was applied for the quantification of quercetin in NLCD. The quercetin peak was completely resolved without any disturbances from degrading products and additives. The developed HPLC method was found to be specific, sensitive, and adequate for the regular assay of marketed products, and NLCD nanoformulation containing quercetin.","PeriodicalId":13751,"journal":{"name":"International Journal of Ayurvedic Medicine","volume":null,"pages":null},"PeriodicalIF":0.1000,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Ayurvedic Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47552/ijam.v15i2.4421","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

An attempt has been made to develop stability representing the reverse-phase High-Performance Liquid Chromatography (RP-HPLC) method for quantitative estimation of quercetin Nanostructure liquid crystalline dispersion. HPLC method was developed by C-18 column with the mobile phase comprising of 0.4% orthophosphoric acid [OPA]: Acetonitrile [ACN] (45:55, v/v), isocratically pumped with a rate of flow 1 mL/min and 255 nm detection wavelength. The method validation of HPLC parameters and degradation tests was analysed by International Conference on Harmonization (ICH) guidelines. The developed HPLC method was found to be specific, linear (r2>0.999) over the selected range of concentration 2-14μg/mL, precise, with the limit of detection (LOD) and limit of quantification (LOQ) as 0.021 and 0.064μg/mL respectively. The entrapment efficiency of quercetin for prepared Nanostructure Liquid Crystalline Dispersion (NLCD) was observed to be 94.55 %. The developed HPLC method was applied for the quantification of quercetin in NLCD. The quercetin peak was completely resolved without any disturbances from degrading products and additives. The developed HPLC method was found to be specific, sensitive, and adequate for the regular assay of marketed products, and NLCD nanoformulation containing quercetin.

查看原文本刊更多论文

建立纳米结构液晶分散体中槲皮素含量的稳定性指示 RP-HPLC 方法

本研究试图开发一种代表反相高效液相色谱法（RP-HPLC）稳定性的方法，用于定量评估槲皮素纳米结构液晶分散体。高效液相色谱法采用 C-18 色谱柱，流动相为 0.4%正磷酸[OPA]：流动相为 0.4%正磷酸[OPA]：乙腈[ACN]（45:55, v/v），流速为 1 mL/min，检测波长为 255 nm。根据国际协调会议（ICH）指南，对高效液相色谱参数和降解试验进行了方法验证。所建立的高效液相色谱法在 2-14μg/mL 的选定浓度范围内具有特异性、线性（r2>0.999）、精确性，检出限（LOD）和定量限（LOQ）分别为 0.021 和 0.064μg/mL。制备的纳米结构液晶分散液中槲皮素的包埋效率为 94.55%。采用所开发的高效液相色谱法对 NLCD 中的槲皮素进行了定量。槲皮素峰完全解析，没有受到降解产物和添加剂的干扰。所建立的高效液相色谱法特异性强、灵敏度高，可用于市售产品和含有槲皮素的 NLCD 纳米制剂的定期检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International Journal of Ayurvedic Medicine MEDICINE, RESEARCH & EXPERIMENTAL-

自引率

50.00%

发文量