Strategies for Increasing the Throughput of Genetic Screening: Lessons Learned from the COVID-19 Pandemic within a University Community

IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BioTech Pub Date : 2024-07-11 DOI:10.3390/biotech13030026
Fernanda Miguel, A. R. Baleizão, A. G. Gomes, Helena Caria, Fátima N. Serralha, Marta C. Justino
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引用次数: 0

Abstract

Amidst the COVID-19 pandemic, the Polytechnic University of Setúbal (IPS) used its expertise in molecular genetics to establish a COVID-19 laboratory, addressing the demand for community-wide testing. Following standard protocols, the IPS COVID Lab received national accreditation in October 2020 and was registered in February 2021. With the emergence of new SARS-CoV-2 variants and safety concerns for students and staff, the lab was further challenged to develop rapid and sensitive diagnostic technologies. Methodologies such as sample-pooling extraction and multiplex protocols were developed to enhance testing efficiency without compromising accuracy. Through Real-Time Reverse Transcription Polymerase Chain Reaction (RT-qPCR) analysis, the effectiveness of sample pooling was validated, proving to be a clear success in COVID-19 screening. Regarding multiplex analysis, the IPS COVID Lab developed an in-house protocol, achieving a sensitivity comparable to that of standard methods while reducing operational time and reagent consumption. This approach, requiring only two wells of a PCR plate (instead of three for samples), presents a more efficient alternative for future testing scenarios, increasing its throughput and testing capacity while upholding accuracy standards. The lessons learned during the SARS-CoV-2 pandemic provide added value for future pandemic situations.
提高基因筛查通量的策略:从大学社区 COVID-19 大流行中汲取的经验教训
在 COVID-19 大流行期间,塞图巴尔理工大学(IPS)利用其在分子遗传学方面的专业知识建立了 COVID-19 实验室,以满足全社区的检测需求。按照标准协议,IPS COVID 实验室于 2020 年 10 月获得国家认证,并于 2021 年 2 月注册。随着 SARS-CoV-2 新变种的出现以及对学生和教职员工安全的担忧,实验室面临着开发快速、灵敏诊断技术的进一步挑战。为了在不影响准确性的前提下提高检测效率,实验室开发了样本池提取和多重方案等方法。通过实时反转录聚合酶链反应(RT-qPCR)分析,验证了样本池提取的有效性,证明在 COVID-19 筛查中取得了明显的成功。在多重分析方面,IPS COVID 实验室开发了一种内部方案,实现了与标准方法相当的灵敏度,同时减少了操作时间和试剂消耗。这种方法只需要 PCR 板的两个孔(而不是样品的三个孔),为今后的检测方案提供了一个更有效的选择,在坚持准确性标准的同时提高了吞吐量和检测能力。在 SARS-CoV-2 大流行期间吸取的经验教训为未来的大流行情况提供了附加值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BioTech
BioTech Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
3.70
自引率
0.00%
发文量
51
审稿时长
11 weeks
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