Curating BOLD records via Bayesian phylogenetic assignments enables harmonization of regional subgeneric classifications and cryptic species detection within the genus Tachina (Diptera: Tachinidae)

IF 3 3区农林科学 Q1 ENTOMOLOGY

Annals of The Entomological Society of America Pub Date : 2024-07-12 DOI:10.1093/aesa/saae018

Frederik Stein, C. C. M. Moura, Oliver Gailing

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引用次数: 0

Abstract

DNA barcoding is a popular tool for species identification of insect samples, with important applications for plant protection. However, Barcode of Life Database queries related to tachinid samples can produce ambiguous results due to contradictory species assignments. Therefore, we applied Bayesian phylogenetic inference to 811 Tachina Meigen, 1803 COI-5P sequences containing all contradictory species assignments of Tachina Barcode Index Numbers. Based on phylogenetic assignment, the assemble species by automatic partitioning (ASAP) analysis, the MonoPhy test and analyses of genetic divergence, we identified 805 sequences representing species of the Tachina genus, whereas six sequences were incorrectly assigned to Tachina. The dataset contained 21 described species and at least seven unidentified species. Additionally, we found that the described species Tachina marklini Zetterstedt, 1838, includes two cryptic species based on ASAP partitioning of sequences, phylogenetic inference and geographical metadata. Furthermore, we harmonized conflicting subgeneric classifications of the Palearctic and Nearctic by employing different subgenera assignments from both partitions. We aimed at monophyletic subgenera partitions. We largely confirmed the validity of the Palearctic Tachina subgenera and Nowickia as a subgenus of Tachina. However, Palearctic Nowickia species formerly allocated to the outdated genus Fabriciella appeared to constitute one monophyletic group with the subgenus Rhachogaster. We conclude that this set of bioinformatics tools is suitable for curating COI-5P-based DNA barcode libraries. Although, curation is limited due to possible species introgression, horizontal gene transfer, incomplete lineage sorting and human errors the resulting dataset enabled the revision of subgenera concepts and the unambiguous cryptic species detection.

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通过贝叶斯系统发生学分配来整理 BOLD 记录，从而统一了 Tachina 属（双翅目：Tachinidae）内的区域亚属分类和隐性物种检测

DNA 条形码是昆虫样本物种鉴定的常用工具，在植物保护方面有重要应用。然而，由于物种分配相互矛盾，生命条形码数据库中有关鹟类样本的查询可能会产生含糊不清的结果。因此，我们应用贝叶斯系统发育推断法对 811 个 Tachina Meigen、1803 COI-5P 序列进行了分析，这些序列包含了 Tachina 条形码索引号中所有相互矛盾的物种分配。根据系统进化分配、自动分区（ASAP）分析、MonoPhy 检验和遗传差异分析，我们确定了 805 条序列代表 Tachina 属的物种，而有 6 条序列被错误地分配到 Tachina 属。数据集中包含 21 个已描述的物种和至少 7 个未确定的物种。此外，根据 ASAP 序列分区、系统发生推断和地理元数据，我们发现已描述的物种 Tachina marklini Zetterstedt, 1838 包括两个隐性物种。此外，我们还协调了古北区和近北区相互冲突的亚属分类，采用了两个分区中不同的亚属归属。我们的目标是单系亚属分区。我们基本上确认了古北 Tachina 亚属和 Nowickia 作为 Tachina 亚属的有效性。然而，以前归属于过时的 Fabriciella 属的古北 Nowickia 物种似乎与 Rhachogaster 亚属构成了一个单系群。我们的结论是，这套生物信息学工具适用于整理基于 COI-5P 的 DNA 条形码库。尽管由于可能存在的物种引入、水平基因转移、不完整的品系分类和人为错误，整理工作受到了限制，但由此产生的数据集使得亚属概念的修正和隐性物种的明确检测成为可能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of The Entomological Society of America 生物-昆虫学

CiteScore

4.90

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Annals of the Entomological Society of America exists to stimulate interdisciplinary dialogue across the entomological disciplines and to advance cooperative interaction among diverse groups of entomologists. It seeks to attract and publish cutting-edge research, reviews, collections of articles on a common topic of broad interest, and discussion of topics with national or international importance. We especially welcome articles covering developing areas of research, controversial issues or debate, and topics of importance to society. Manuscripts that are primarily reports of new species, methodology, pest management, or the biology of single species generally will be referred to other journals of the ESA. The most important criteria for acceptance are quality of work and breadth of interest to the readership.