High Burden of Carbapenem-Resistant Organisms Screened by Xpert Carba-R Assay Versus Culture from Intensive Care Patients in Indonesia

IF 0.7 Q4 MICROBIOLOGY
Mohammad Agung Marzah, F. S. Widyatama, Rosantia Sarassari, W. Setyarini, Alicia Margaretta Widya, Sulikah Sulikah, B. Semedi, P. Airlangga, K. Kuntaman, Toshiro Shirakawa, E. Koendhori
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引用次数: 0

Abstract

The World Health Organization (WHO) considers carbapenem-resistant organisms (CROs) to be critical-level pathogens. Regular screening for high-risk CRO colonization is essential, especially in the ICU. Direct detection of carbapenem-resistant genes is possible using the FDA-approved Xpert Carba-R assay. This study evaluated its reliability compared with the culture technique at a tertiary hospital in Indonesia. A high number of CRO colonization was found using the culture technique and the Xpert Carba-R assay with about 31 and 26 positive results out of 100 total samples, respectively. Both methods detected blaNDM in 11 samples, and the Xpert Carba-R assay detected one sample co-presenting with blaVIM that was not detected by PCR. The Xpert Carba-R assay did not detect the gene in 73 samples following negative results with the culture technique. Fifteen samples were detected gene by the Xpert Carba-R assay though there was no gene by the culture method, showing that the Xpert Carba-R assay demonstrated a high degree of sensitivity in identifying carbapenem-resistance genes. Carbapenem-resistance genes common in Indonesia other than those examined by Xpert Carba-R assay in this study (i.e., blaOXA-23 and blaOXA-24) or non-enzymatic mechanisms may also produce resistance in many colonies without the examined genes. Finally, the Xpert Carba-R assay produced faster findings than the culture technique.
印度尼西亚重症监护患者中经 Xpert Carba-R 检测法与培养法筛查出的耐碳青霉烯类病菌的高负担率
世界卫生组织(WHO)将耐碳青霉烯类生物(CRO)视为临界病原体。定期筛查高风险的 CRO 定植至关重要,尤其是在重症监护病房。使用美国食品及药物管理局(FDA)批准的 Xpert Carba-R 检测法可直接检测耐碳青霉烯类基因。本研究在印度尼西亚的一家三甲医院评估了其与培养技术相比的可靠性。使用培养技术和 Xpert Carba-R 检测法发现了大量的 CRO 定植,在 100 份样本中分别有 31 份和 26 份阳性结果。两种方法都检测出了 11 个样本中的 blaNDM,Xpert Carba-R 检测法检测出了一个样本中同时存在 BlaVIM,而 PCR 检测法没有检测到。在 73 个样本中,Xpert Carba-R 检测法没有检测到该基因,而培养技术检测结果为阴性。有 15 个样本用 Xpert Carba-R 检测法检测到了耐碳青霉烯基因,而用培养法则没有检测到该基因,这表明 Xpert Carba-R 检测法在识别耐碳青霉烯基因方面具有很高的灵敏度。印尼常见的碳青霉烯类耐药基因(如 blaOXA-23 和 blaOXA-24)与本研究中 Xpert Carba-R 检测法检测的基因不同,也可能在许多没有检测基因的菌落中产生耐药性。最后,与培养技术相比,Xpert Carba-R 检测法能更快地得出结论。
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来源期刊
Journal of Pure and Applied Microbiology
Journal of Pure and Applied Microbiology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
2.00
自引率
0.00%
发文量
266
审稿时长
11 months
期刊介绍: Journal of Pure and Applied Microbiology (JPAM) is a peer-reviewed, open access international journal of microbiology aims to advance and disseminate research among scientists, academics, clinicians and microbiologists around the world. JPAM publishes high-quality research in all aspects of microbiology in both online and print form on quarterly basis.
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