Chloride intracellular channel 4 participates in the regulation of lipopolysaccharide-induced inflammatory responses in human bronchial epithelial cells

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-17 DOI:10.1016/j.resp.2024.104303

Jinhua Luo , Jia Wang , Huijun Liu , Wang Jiang , Lang Pan , Wenjie Huang , Caixia Liu , Xiangping Qu , Chi Liu , Xiaoqun Qin , Yang Xiang

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Abstract

The airway epithelium is located at the interactional boundary between the external and internal environments of the organism and is often exposed to harmful environmental stimuli. Inflammatory response that occurs after airway epithelial stress is the basis of many lung and systemic diseases. Chloride intracellular channel 4 (CLIC4) is abundantly expressed in epithelial cells. The purpose of this study was to investigate whether CLIC4 is involved in the regulation of lipopolysaccharide (LPS)-induced inflammatory response in airway epithelial cells and to clarify its potential mechanism. Our results showed that LPS induced inflammatory response and decreased CLIC4 levels in vivo and in vitro. CLIC4 silencing aggravated the inflammatory response in epithelial cells, while overexpression of CLIC4 combined with LPS exposure significantly decreased the inflammatory response compared with cells exposed to LPS without CLIC4 overexpression. By labeling intracellular chloride ions with chloride fluorescent probe MQAE, we showed that CLIC4 mediated intracellular chloride ion-regulated LPS-induced cellular inflammatory response.

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细胞内氯离子通道 4 参与调节脂多糖诱导的人类支气管上皮细胞炎症反应

气道上皮位于机体内外环境相互作用的边界，经常暴露在有害的环境刺激下。气道上皮受压后发生的炎症反应是许多肺部和全身疾病的基础。细胞内氯离子通道 4（CLIC4）在上皮细胞中大量表达。本研究旨在探讨 CLIC4 是否参与调控脂多糖（LPS）诱导的气道上皮细胞炎症反应，并阐明其潜在机制。我们的研究结果表明，LPS诱导了炎症反应，并降低了体内和体外CLIC4的水平。沉默 CLIC4 会加重上皮细胞的炎症反应，而与暴露于 LPS 但未过表达 CLIC4 的细胞相比，过表达 CLIC4 并暴露于 LPS 的细胞会显著降低炎症反应。通过用氯离子荧光探针 MQAE 标记细胞内氯离子，我们发现 CLIC4 介导了细胞内氯离子调节 LPS 诱导的细胞炎症反应。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.