The geogrid-soil interface characteristic is a critical factor influencing the behavior of reinforced soil structures. In seasonal frozen regions, the shear strength of the geogrid-soil interface fluctuates periodically with temperature, necessitating consideration of freeze-thaw cycle effects during engineering design. In this study, a series of large-scale direct shear tests were conducted to investigate the geogrid-sand interface behavior under different freeze-thaw cycles. The mechanism of the evolution and variation of the geogrid-sand interaction was discussed based on test results and the associated mesoscopic analysis. The results indicate that freezing enhances the shear strength of the geogrid-sand interface, whereas freeze-thaw cycles reduce the geogrid-sand interface shear stress. The cohesion and friction angle of the geogrid-sand interface decreased as the number of freeze-thaw cycles increased, but tended to stabilize after several freeze-thaw cycles. The influence of freeze-thaw cycles on the tensile strength and elongation of the geogrid was insignificant. Internal changes in sand during freeze-thaw cycles were considered as the key issue that led to the deterioration of the geogrid-sand interface. Furthermore, considering the interface cohesion and normal stress, the influence of freeze-thaw cycles on the interaction coefficient k between geogrid and soil was analyzed, which is referable for the design and application of reinforced soil engineering in cold regions under similar conditions.