{"title":"Exploring the diagnostic potential of immunoglobulin A-microbiota interplay in liver cirrhosis and spontaneous bacterial peritonitis.","authors":"Liang-Jie Zhang, Wen-Qi Huang, Yuan Zhang, You-Lian Zhou, Hao-Ming Xu, Chong Zhao, Yu-Qiang Nie","doi":"10.1002/kjm2.12876","DOIUrl":null,"url":null,"abstract":"<p><p>The human gut microbiota significantly impacts health, including liver conditions like liver cirrhosis (LC) and spontaneous bacterial peritonitis (SBP). Immunoglobulin A (IgA) plays a central role in maintaining gut microbial balance. Understanding IgA's interplay with gut microbiota and liver health is crucial. This study explores the relationship between fecal IgA levels, gut microbiota, and liver injury severity. A total of 69 LC patients and 30 healthy controls were studied. Fecal IgA levels were measured using ELISA, and IgA-coated bacteria were quantified via flow cytometry. Microbiota diversity and composition were assessed through 16S rRNA sequencing. Liver injury severity was graded using the Child-Pugh score. Statistical analyses determined correlations. LC patients had higher fecal IgA levels than controls, correlating positively with liver injury severity. Microbiota diversity decreased with severity, accompanied by shifts in composition favoring pro-inflammatory species. Ralstonia abundance positively correlated with liver injury, whereas Faecalibacterium showed a negative correlation. Specific microbial markers for SBP were identified. Functional profiling revealed altered microbial functionalities in LC and SBP. Elevated fecal IgA levels, coupled with microbiota alterations, correlate with liver injury severity in LC patients. Modulating gut microbiota could be a promising strategy for managing liver-related conditions. Further research is needed to understand underlying mechanisms and translate findings into clinical practice, potentially improving patient outcomes.</p>","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"837-851"},"PeriodicalIF":0.0000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Kaohsiung journal of medical sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/kjm2.12876","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/18 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The human gut microbiota significantly impacts health, including liver conditions like liver cirrhosis (LC) and spontaneous bacterial peritonitis (SBP). Immunoglobulin A (IgA) plays a central role in maintaining gut microbial balance. Understanding IgA's interplay with gut microbiota and liver health is crucial. This study explores the relationship between fecal IgA levels, gut microbiota, and liver injury severity. A total of 69 LC patients and 30 healthy controls were studied. Fecal IgA levels were measured using ELISA, and IgA-coated bacteria were quantified via flow cytometry. Microbiota diversity and composition were assessed through 16S rRNA sequencing. Liver injury severity was graded using the Child-Pugh score. Statistical analyses determined correlations. LC patients had higher fecal IgA levels than controls, correlating positively with liver injury severity. Microbiota diversity decreased with severity, accompanied by shifts in composition favoring pro-inflammatory species. Ralstonia abundance positively correlated with liver injury, whereas Faecalibacterium showed a negative correlation. Specific microbial markers for SBP were identified. Functional profiling revealed altered microbial functionalities in LC and SBP. Elevated fecal IgA levels, coupled with microbiota alterations, correlate with liver injury severity in LC patients. Modulating gut microbiota could be a promising strategy for managing liver-related conditions. Further research is needed to understand underlying mechanisms and translate findings into clinical practice, potentially improving patient outcomes.
人体肠道微生物群对健康有重大影响,包括肝硬化(LC)和自发性细菌性腹膜炎(SBP)等肝脏疾病。免疫球蛋白 A (IgA) 在维持肠道微生物平衡方面发挥着核心作用。了解 IgA 与肠道微生物群和肝脏健康之间的相互作用至关重要。本研究探讨了粪便 IgA 水平、肠道微生物群和肝损伤严重程度之间的关系。共研究了 69 名肝癌患者和 30 名健康对照组。采用酶联免疫吸附法测定粪便 IgA 水平,并通过流式细胞术对 IgA 包裹细菌进行定量。通过 16S rRNA 测序评估微生物群的多样性和组成。肝损伤严重程度采用 Child-Pugh 评分进行分级。统计分析确定了相关性。LC 患者的粪便 IgA 水平高于对照组,与肝损伤严重程度呈正相关。微生物群的多样性随着严重程度的增加而减少,同时微生物群的组成偏向于促炎菌种。Ralstonia丰度与肝损伤呈正相关,而粪杆菌则呈负相关。确定了 SBP 的特定微生物标记。功能分析表明,LC 和 SBP 中的微生物功能发生了改变。粪便 IgA 水平的升高与微生物群的改变与 LC 患者肝损伤的严重程度相关。调节肠道微生物群可能是治疗肝脏相关疾病的一种有前途的策略。要了解潜在的机制并将研究结果转化为临床实践,从而改善患者的预后,还需要进一步的研究。