André Henrique de Oliveira, Mayura Marques Magalhães Rubinger, Anderson da Silva Rabello, Nathália Matias Albuini-Oliveira, Antonio Eustáquio Carneiro Vidigal, Marcelo Ribeiro Leite de Oliveira, Eder do Couto Tavares, José Eduardo Serrão
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引用次数: 0
Abstract
Apis mellifera, crucial pollinators for both native and cultivated plants, also yield various products such as honey, wax, royal jelly, and propolis, extensively utilized in the food, pharmaceuticals, and cosmetics industries. Nosema ceranae, a prevalent microsporidian worldwide, stands as a significant pathogen for A. mellifera, showing resistance to conventional antibiotics. Consequently, the exploration of novel compounds for N. ceranae control becomes imperative. Dithiocarbimate derivatives emerge as promising antifungal candidates under evaluation for combating various pathogens, particularly those affecting plants. This study assessed the toxicity profile of six dithiocarbimate derivatives on A. mellifera worker survival and N. ceranae pathogen. Among these, four compounds exhibited minimal bee mortality and proceeded to further evaluation against N. ceranae. In vitro assays demonstrated their inhibitory effects on spore germination. Remarkably, the most potent compound suppressed N. ceranae spores by 62% at a concentration of 20 µmol L-1in vivo. Thus, these dithiocarbimate derivatives represent promising new antifungal agents for combatting nosemosis in honey bee populations.
蜜蜂是本地植物和栽培植物的重要授粉者,还生产蜂蜜、蜡、蜂王浆和蜂胶等各种产品,这些产品被广泛用于食品、医药和化妆品行业。陶瓷鼻疽(Nosema ceranae)是一种全球流行的微孢子虫,是蜜蜂的重要病原体,对传统抗生素具有抗药性。因此,探索新型化合物来控制 N. ceranae 变得势在必行。二硫代碳酸酯衍生物作为有前途的抗真菌候选化合物,正在被评估用于防治各种病原体,尤其是影响植物的病原体。本研究评估了六种二硫代碳酸酯衍生物对 A. mellifera 工蜂存活率和 N. ceranae 病原体的毒性。其中,有四种化合物的蜜蜂死亡率极低,并进一步评估了它们对 N. ceranae 的毒性。体外试验证明了它们对孢子萌发的抑制作用。值得注意的是,在体内浓度为 20 µmol L-1 时,最有效的化合物对 N. ceranae 孢子的抑制率为 62%。因此,这些二硫代碳酸酯衍生物是很有前途的抗真菌新药,可用于防治蜜蜂的鼻疽病。
期刊介绍:
AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.