Green light mediates atypical photomorphogenesis by dual modulation of Arabidopsis phytochromes B and A

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Miqi Xu, Yi-Yuan Wang, Yujie Wu, Xiuhong Zhou, Ziyan Shan, Kunying Tao, Kaiqiang Qian, Xuncheng Wang, Jian Li, Qingqing Wu, Xing Wang Deng, Jun-Jie Ling
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Abstract

Although green light (GL) is located in the middle of the visible light spectrum and regulates a series of plant developmental processes, the mechanism by which it regulates seedling development is largely unknown. In this study, we demonstrated that GL promotes atypical photomorphogenesis in Arabidopsis thaliana via the dual regulations of phytochrome B (phyB) and phyA. Although the Pr-to-Pfr conversion rates of phyB and phyA under GL were lower than those under red light (RL) in a fluence rate-dependent and time-dependent manner, long-term treatment with GL induced high Pfr/Pr ratios of phyB and phyA. Moreover, GL induced the formation of numerous small phyB photobodies in the nucleus, resulting in atypical photomorphogenesis, with smaller cotyledon opening angles and longer hypocotyls in seedlings compared to RL. The abundance of phyA significantly decreased after short- and long-term GL treatments. We determined that four major PHYTOCHROME-INTERACTING FACTORs (PIFs: PIF1, PIF3, PIF4, and PIF5) act downstream of phyB in GL-mediated cotyledon opening. In addition, GL plays opposite roles in regulating different PIFs. For example, under continuous GL, the protein levels of all PIFs decreased, whereas the transcript levels of PIF4 and PIF5 strongly increased compared with dark treatment. Taken together, our work provides a detailed molecular framework for understanding the role of the antagonistic regulations of phyB and phyA in GL-mediated atypical photomorphogenesis.

Abstract Image

绿光通过对拟南芥植物色素 B 和 A 的双重调节介导非典型光形态发生。
尽管绿光(GL)位于可见光光谱的中间并调控着一系列植物发育过程,但其调控幼苗发育的机制在很大程度上仍是未知的。本研究证明,绿光通过植物色素 B(phyB)和 phyA 的双重调控促进拟南芥的非典型光形态发生。虽然GL条件下phyB和phyA的Pr-Pfr转化率低于红光(RL)条件下的Pr-Pfr转化率,但GL的长期处理诱导了phyB和phyA的高Pfr/Pr比。此外,GL 还诱导细胞核中形成大量 phyB 小光体,导致非典型光形态发生,与 RL 相比,幼苗的子叶开口角度更小,下胚轴更长。经过短期和长期的 GL 处理后,phyA 的丰度明显下降。我们确定,在 GL 介导的子叶打开过程中,四种主要的植物互作因子(PIFs:PIF1、PIF3、PIF4 和 PIF5)作用于 phyB 的下游。此外,GL 对不同的 PIF 起着相反的调控作用。例如,与黑暗处理相比,在连续 GL 条件下,所有 PIFs 的蛋白水平均下降,而 PIF4 和 PIF5 的转录物水平则强烈上升。综上所述,我们的研究为理解phyB和phyA在GL介导的非典型光形态发生中的拮抗调控作用提供了一个详细的分子框架。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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