Expression analysis of thg1l during Xenopus laevis development.

Davide Martini, Chiara De Cesari, Matteo Digregorio, Alessia Muscò, Guido Giudetti, Martina Giannaccini, Massimiliano Andreazzoli
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Abstract

The tRNA-histidine guanylyltransferase 1-like (THG1L), also known as induced in high glucose-1 (IHG-1), encodes for an essential mitochondria-associated protein highly conserved throughout evolution, that catalyses the 3'-5' addition of a guanine to the 5'-end of tRNA-histidine (tRNAHis). Previous data indicated that THG1L plays a crucial role in the regulation of mitochondrial biogenesis and dynamics, in ATP production, and is critically involved in the modulation of apoptosis, cell-cycle progression and survival, as well as in cellular stress responses and redox homeostasis. Dysregulations of THG1L expression play a central role in various pathologies, including nephropathies, and neurodevelopmental disorders often characterized by developmental delay and cerebellar ataxia. Despite the essential role of THG1L, little is known about its expression during vertebrate development. Herein, we examined the detailed spatio-temporal expression of this gene in the developing Xenopus laevis. Our results show that thg1l is maternally inherited and its temporal expression suggests a role during the earliest stages of embryogenesis. Spatially, thg1l mRNA localizes in the ectoderm and marginal zone mesoderm during early stages of development. Then, at tadpole stages, thg1l transcripts mostly localise in neural crests and their derivatives, somites, developing kidney and central nervous system, therefore largely coinciding with territories displaying intense energy metabolism during organogenesis in Xenopus.

thg1l在爪蟾发育过程中的表达分析。
tRNA-histidine guanylyltransferase 1-like (THG1L),又称高糖诱导-1 (IHG-1),编码一种在整个进化过程中高度保守的重要线粒体相关蛋白,它能催化鸟嘌呤在 tRNA-histidine (tRNAHis)的 5'-5' 端进行 3'-5' 添加。以前的数据表明,THG1L 在线粒体生物生成和动态调控、ATP 生成中发挥着关键作用,并在细胞凋亡、细胞周期进展和存活调控以及细胞应激反应和氧化还原平衡中发挥着重要作用。THG1L 表达失调在各种病症中起着核心作用,包括肾病和通常以发育迟缓和小脑共济失调为特征的神经发育疾病。尽管THG1L起着至关重要的作用,但人们对其在脊椎动物发育过程中的表达却知之甚少。在此,我们研究了该基因在发育中的爪蟾中的详细时空表达。我们的研究结果表明,thg1l 是母性遗传的,它在时间上的表达表明它在胚胎发生的最早期阶段发挥作用。在空间上,thg1l mRNA 在发育早期定位于外胚层和边缘区中胚层。然后,在蝌蚪阶段,thg1l转录本主要定位于神经嵴及其衍生物、体节、发育中的肾脏和中枢神经系统,因此在很大程度上与章鱼器官形成过程中能量代谢旺盛的区域相吻合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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