{"title":"A Ratiometric Fast-Response Fluorescent Probe Based on Dicyanoisophorone for Monitoring HClO in Paper Test Strips and Living Mice.","authors":"Zhenzhen Wang, Ziqing Ye, Yumiao Sheng, Kedian Xu, Ruiqing Liang, Yunling Gao","doi":"10.1007/s10895-024-03837-z","DOIUrl":null,"url":null,"abstract":"<p><p>A new dicyanoisophorone-based ratiometric fluorescent probe NOSA was synthesized and characterized. It showed a fast fluorescence response to HClO with the emission color change from dark green to bright red. NMR, IR, and HRMS suggested that the detection of NOSA to HClO may originate from the hydroxyl deprotection reaction by HClO on the molecule NOSA, which caused a red-shift of fluorescence. The probe NOSA displayed high selectivity and excellent anti-interference performance with a limit of detection at 3.835 × 10<sup>-7</sup> M. The convenient paper test strips were successfully obtained and applied to the detection of HClO based on fluorescence color change with the varied NaClO concentration. Moreover, spiked recovery experiments in real water samples indicated that the probe NSOA could quantitatively detect HClO, and the fluorescence bio-imagings in vivo were carried out, and HClO detection in biosystems using NOSA was realized.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":"4419-4429"},"PeriodicalIF":3.1000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fluorescence","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10895-024-03837-z","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/17 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
A new dicyanoisophorone-based ratiometric fluorescent probe NOSA was synthesized and characterized. It showed a fast fluorescence response to HClO with the emission color change from dark green to bright red. NMR, IR, and HRMS suggested that the detection of NOSA to HClO may originate from the hydroxyl deprotection reaction by HClO on the molecule NOSA, which caused a red-shift of fluorescence. The probe NOSA displayed high selectivity and excellent anti-interference performance with a limit of detection at 3.835 × 10-7 M. The convenient paper test strips were successfully obtained and applied to the detection of HClO based on fluorescence color change with the varied NaClO concentration. Moreover, spiked recovery experiments in real water samples indicated that the probe NSOA could quantitatively detect HClO, and the fluorescence bio-imagings in vivo were carried out, and HClO detection in biosystems using NOSA was realized.
期刊介绍：
Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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