Enhancing high-efficient cadmium biosorption of Escherichia coli via cell surface displaying metallothionien CUP1.

IF 2.2 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Environmental Technology Pub Date : 2025-03-01 Epub Date: 2024-07-17 DOI:10.1080/09593330.2024.2375006
Nan He, Ziru Wang, Ling Lei, Changxuan Chen, Yixian Qin, Jingxiang Tang, Kecheng Dai, Heng Xu
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引用次数: 0

Abstract

Cadmium (Cd) is one of the common heavy metal pollutants in soil, which can induce various diseases and pose a serious threat to human health. Metallothioneins (MTs) are well-known for their excellent metal binding ability due to a high content of cysteine, which has great potential for heavy metal chelation. In this study, we used the Escherichia coli (E. coli) surface display system LPP-OmpA to construct a recombinant plasmid pBSD-LCF encoding LPP-OmpA-CUP1-Flag fusion protein. Then we displayed the metallothionein CUP1 from Saccharomyces cerevisiae on E. coli DH5α surface for Cd removing. The feasibility of surface display of metallothionein CUP1 in recombinant E. coli DH5α (pBSD-LCF) by Lpp-OmpA system was proved by flow cytometry and western blot analysis, and the specificity of the fusion protein in the recombinant strain was also verified. The results showed that the Cd2+ resistance capacity of DH5α (pBSD-LCF) was highly enhanced by about 200%. Fourier-transform infrared spectroscopy showed that sulfhydryl and sulfonyl groups were involved in Cd2+ binding to cell surface of DH5α (pBSD-LCF). Meanwhile, Cd removal rate by DH5α (pBSD-LCF) was promoted to 95.2%. Thus, the recombinant strain E. coli DH5α (pBSD-LCF) can effectively chelate environmental metals, and the cell surface expression of metallothionein on E. coli can provide new ideas and directions for heavy metals remediation.

通过细胞表面显示金属硫蛋白 CUP1,增强大肠杆菌对镉的高效生物吸附。
镉(Cd)是土壤中常见的重金属污染物之一,可诱发多种疾病,严重威胁人类健康。众所周知,金属硫蛋白(MTs)因含有大量半胱氨酸而具有出色的金属结合能力,在重金属螯合方面具有巨大潜力。在本研究中,我们利用大肠杆菌(E. coli)表面展示系统 LPP-OmpA 构建了编码 LPP-OmpA-CUP1-Flag 融合蛋白的重组质粒 pBSD-LCF。然后,我们将来自酿酒酵母的金属硫蛋白 CUP1 展示在大肠杆菌 DH5α 表面,以去除镉。通过流式细胞术和Western印迹分析,证明了金属硫蛋白CUP1在重组大肠杆菌DH5α(pBSD-LCF)中通过Lpp-OmpA系统进行表面展示的可行性,并验证了该融合蛋白在重组菌株中的特异性。结果表明,DH5α(pBSD-LCF)对Cd2+的抗性增强了约200%。傅立叶变换红外光谱显示,巯基和磺酰基参与了 Cd2+ 与 DH5α (pBSD-LCF)细胞表面的结合。同时,DH5α(pBSD-LCF)对镉的去除率提高到了 95.2%。因此,重组菌株大肠杆菌 DH5α (pBSD-LCF)能有效螯合环境中的金属,而金属硫蛋白在大肠杆菌细胞表面的表达能为重金属修复提供新的思路和方向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Environmental Technology
Environmental Technology 环境科学-环境科学
CiteScore
6.50
自引率
3.60%
发文量
0
审稿时长
4 months
期刊介绍: Environmental Technology is a leading journal for the rapid publication of science and technology papers on a wide range of topics in applied environmental studies, from environmental engineering to environmental biotechnology, the circular economy, municipal and industrial wastewater management, drinking-water treatment, air- and water-pollution control, solid-waste management, industrial hygiene and associated technologies. Environmental Technology is intended to provide rapid publication of new developments in environmental technology. The journal has an international readership with a broad scientific base. Contributions will be accepted from scientists and engineers in industry, government and universities. Accepted manuscripts are generally published within four months. Please note that Environmental Technology does not publish any review papers unless for a specified special issue which is decided by the Editor. Please do submit your review papers to our sister journal Environmental Technology Reviews at http://www.tandfonline.com/toc/tetr20/current
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