Adhesion of Candida albicans on PTFE membranes used in guided bone regeneration

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2024-07-16 DOI:10.1002/cre2.902

Adel Al-Asfour, Maribasappa Karched, Syed Saad Bin Qasim, Gregor-Georg Zafiropoulos

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引用次数: 0

Abstract

Objectives

Guided bone regeneration (GBR) is a core procedure used to regenerate bone defects. The aim of the study was to investigate the adherence of Candida albicans on six commercially available polytetrafluoroethylene (PTFE) membranes used in GBR procedures and the subsequent clinical consequences.

Materials and Methods

Six commercially available PTFE membranes were tested. Two of the membranes had a textured surface and the other four a plane, nontextured one. C. albicans (ATCC 24433) was cultured for 24 h, and its cell surface hydrophobicity was assessed using a modified method. C. albicans adhesion to membrane discs was studied by scanning electron microscopy (SEM) and real-time polymerase chain reaction (PCR).

Results

C. albicans was found to be hydrophobic (77.25%). SEM analysis showed that C. albicans adherence to all membranes examined was characterized by patchy, scattered, and small clustered patterns except for one nontextured membrane with a most rough surface in which a thick biofilm was observed. Real-time PCR quantification revealed significantly greater adhesion of C. albicans cells to PTFE membranes than the control membrane (p ≤ .001) with the membranes having a textured surface exhibiting the highest count of 2680 × 10⁴ cells/ml compared to the count of 707 × 10⁴ cells/mL on those with a nontextured one (p ≤ .001). One membrane with nontextured surface, but with most rough surface was found to exhibit the highest count of 3010 × 10⁴ cells/ml (p ≤ .05).

Conclusion

The results of this study indicate that C. albicans adhesion on membranes' surfaces depends on the degree of surface roughness and/or on the presence of a texture. Textured PTFE membranes and/or membranes high roughness showed significantly more adhered C. albicans cells. These findings can impact the surgeon's choice of GBR membrane and postoperative maintenance.

Abstract Image

查看原文本刊更多论文

白色念珠菌在用于引导骨再生的聚四氟乙烯膜上的粘附。

目的：引导骨再生（GBR）是用于骨缺损再生的核心程序。本研究旨在调查白念珠菌在 GBR 程序中使用的六种市售聚四氟乙烯（PTFE）膜上的附着情况以及随后的临床后果：测试了六种市售聚四氟乙烯膜。材料：对六种市售聚四氟乙烯膜进行了测试，其中两种膜的表面有纹理，另外四种膜的表面平整无纹理。将白僵菌（ATCC 24433）培养 24 小时后，使用改进的方法评估其细胞表面疏水性。通过扫描电子显微镜（SEM）和实时聚合酶链反应（PCR）研究了白僵菌对膜盘的粘附情况：结果：发现白僵菌具有疏水性（77.25%）。扫描电镜分析表明，白僵菌粘附在所有受检膜上的特点是斑块状、分散状和小团块状，只有一种表面最粗糙的无纹理膜上出现了厚厚的生物膜。实时 PCR 定量显示，白僵菌细胞在聚四氟乙烯膜上的附着力明显高于对照膜（p ≤ .001），表面有纹理的膜上的细胞数最高，为 2680 × 104 个/毫升，而表面无纹理的膜上的细胞数为 707 × 104 个/毫升（p ≤ .001）。发现表面无纹理但最粗糙的一层膜的细胞数最多，达到 3010 × 104 个/毫升（p ≤ .05）：本研究结果表明，白僵菌在膜表面的粘附取决于表面粗糙度和/或纹理的存在。有纹理的聚四氟乙烯膜和/或高粗糙度膜上粘附的白僵菌细胞明显较多。这些发现会影响外科医生对 GBR 膜的选择和术后维护。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.