Ginkgetin Pretreatment Reduces Inflammatory Response in DCD Donor Liver via JAK2/STAT3 Signaling Pathway.

IF 1.1 4区 医学 Q3 SURGERY
Jia Liu, Jiansheng Xiao, Qin Deng, ZhiHui Fu, Qi Xiao
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引用次数: 0

Abstract

BACKGROUND Ginkgetin inhibits growth of tumor cells, reducing blood lipids, and improving atherosclerosis, but the protective effect of ginkgetin in donation after cardiac death (DCD) livers is still unknown. The aim of this study was to determine whether pretreatment of DCD donor livers with ginkgetin can reduce inflammatory response through the JAK2/STAT3 signaling pathway. MATERIAL AND METHODS Twenty male Sprague-Dawley rats (200-250 g) were randomly divided into 4 groups: Sham, DCD, Ginkgetin (0.6 mg/kg) pretreatment 1 h before surgery, and Ginkgetin (0.6 mg/kg) plus broussonin E (0.3 mg/kg) (JAK2/STAT3 signaling agonist) pretreatment 1 h before surgery. Rat livers were subjected to 30 min warm ischemia and 24 h cold storage to simulate the preservation process of DCD donor livers, followed by normothermic machine perfusion for 1 h to simulate liver reperfusion in vivo. Liver tissues and perfusate samples were collected for further studies. RESULTS Ginkgetin pretreatment significantly decreased the values of ALT and AST (P<0.05), and improved histological alterations according to improved Suzuki's Score (P<0.05). Ginkgetin also inhibited the protein expression levels of p-JAK2/JAK2 and p-STAT3/STAT3 (P<0.05). Furthermore, ginkgetin pretreatment inhibited levels of interleukin-1β, interleukin-6 and tumor necrosis factor a (P<0.05) to suppress inflammatory response. In addition, broussonin E reversed the improvement of ginkgetin on DCD donor livers. CONCLUSIONS Ginkgetin can inhibit the inflammatory response through the JAK2/STAT3 signaling pathway to improve the quality of DCD donor livers.

银杏黄酮预处理通过 JAK2/STAT3 信号通路降低 DCD 供体肝脏的炎症反应
背景 银杏黄酮能抑制肿瘤细胞的生长、降低血脂和改善动脉粥样硬化,但银杏黄酮对心脏死亡后捐献(DCD)肝脏的保护作用尚不清楚。本研究旨在确定银杏黄酮预处理 DCD 供体肝脏是否能通过 JAK2/STAT3 信号通路减轻炎症反应。材料和方法 20 只雄性 Sprague-Dawley 大鼠(200-250 克)随机分为 4 组:Sham 组、DCD 组、术前 1 h Ginkgetin(0.6 mg/kg)预处理组和术前 1 h Ginkgetin(0.6 mg/kg)加布鲁索宁 E(0.3 mg/kg)(JAK2/STAT3 信号激动剂)预处理组。对大鼠肝脏进行30分钟温缺血和24小时冷藏,以模拟DCD供体肝脏的保存过程,然后进行1小时常温机器灌注,以模拟体内肝脏再灌注。收集肝脏组织和灌注液样本用于进一步研究。结果 银杏黄酮预处理可明显降低谷丙转氨酶和谷草转氨酶(P
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来源期刊
CiteScore
2.50
自引率
0.00%
发文量
79
审稿时长
>12 weeks
期刊介绍: Annals of Transplantation is one of the fast-developing journals open to all scientists and fields of transplant medicine and related research. The journal is published quarterly and provides extensive coverage of the most important advances in transplantation. Using an electronic on-line submission and peer review tracking system, Annals of Transplantation is committed to rapid review and publication. The average time to first decision is around 3-4 weeks. Time to publication of accepted manuscripts continues to be shortened, with the Editorial team committed to a goal of 3 months from acceptance to publication. Expert reseachers and clinicians from around the world contribute original Articles, Review Papers, Case Reports and Special Reports in every pertinent specialty, providing a lot of arguments for discussion of exciting developments and controversies in the field.
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