Silence of Aminopeptidase N 2 gene reveals the trade-offs for acquiring Cry1Ac resistance in Plutella xylostella

IF 1.5 4区 农林科学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Zhuohong Lv, Shuwen Yu, Yafei Zhao, Zhongxia Yang
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引用次数: 0

Abstract

Bacillus thuringiensis (Bt) is widely used as a biopesticide worldwide. To date, at least eight pest species have been found to be resistant to Bt in the field. As the first pest that was reported having resistance to Bt in the field, considerable research has been done on the mechanisms of Bt resistance in Plutella xylostella. However, whether the acquisition of Bt resistance by P. xylostella comes at a fitness cost is also a valuable question. In this study, Aminopeptidase-N 2 (APN2), a Cry toxin receptor gene of P. xylostella, was knocked down by RNA interference, resulting in improved resistance to Cry1Ac. It was also found that larval mortality of APN2 knockdown P. xylostella was significantly higher than that of the control, while the pupation rate, pupal weight, eclosion rate, fecundity (egg/female), hatchability, and female adult longevity were significantly lower in APN2 knockdown P. xylostella than in the control. These results illustrate that if Cry1Ac resistance was obtained only through the reduction of APN2 expression, P. xylostella would need to incur some fitness costs for it.

氨基肽酶 N 2 基因的沉默揭示了木虱获得 Cry1Ac 抗性的权衡。
苏云金芽孢杆菌(Bt)作为一种生物杀虫剂在全世界被广泛使用。迄今为止,已发现至少有八种害虫对 Bt 产生抗药性。作为第一个被报道在田间对 Bt 产生抗性的害虫,人们对木虱 Plutella 对 Bt 产生抗性的机制进行了大量研究。然而,木虱获得 Bt 抗性是否需要付出适应性代价也是一个有价值的问题。在这项研究中,通过 RNA 干扰敲除了木虱的 Cry 毒素受体基因 Aminopeptidase-N 2 (APN2),从而提高了木虱对 Cry1Ac 的抗性。研究还发现,APN2基因敲除后的木虱幼虫死亡率明显高于对照组,而APN2基因敲除后的木虱化蛹率、蛹重、羽化率、受精率(卵/雌虫)、孵化率和雌成虫寿命则明显低于对照组。这些结果表明,如果仅通过减少 APN2 的表达来获得 Cry1Ac 抗性,木虱需要为此付出一定的适应成本。
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来源期刊
CiteScore
4.30
自引率
4.50%
发文量
115
审稿时长
12 months
期刊介绍: Archives of Insect Biochemistry and Physiology is an international journal that publishes articles in English that are of interest to insect biochemists and physiologists. Generally these articles will be in, or related to, one of the following subject areas: Behavior, Bioinformatics, Carbohydrates, Cell Line Development, Cell Signalling, Development, Drug Discovery, Endocrinology, Enzymes, Lipids, Molecular Biology, Neurobiology, Nucleic Acids, Nutrition, Peptides, Pharmacology, Pollinators, Proteins, Toxicology. Archives will publish only original articles. Articles that are confirmatory in nature or deal with analytical methods previously described will not be accepted.
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