Expression of somatostatin receptors in canine and feline meningioma.

IF 1.8 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Medicine and Science Pub Date : 2024-07-01 DOI:10.1002/vms3.1537

Martin Immler, Michael Wolfram, Anna Oevermann, Ingrid Walter, Birgitt Wolfesberger, Alexander Tichy, Gabriele Gradner

{"title":"Expression of somatostatin receptors in canine and feline meningioma.","authors":"Martin Immler, Michael Wolfram, Anna Oevermann, Ingrid Walter, Birgitt Wolfesberger, Alexander Tichy, Gabriele Gradner","doi":"10.1002/vms3.1537","DOIUrl":null,"url":null,"abstract":"Objectives: The standard treatment for canine and feline meningiomas includes radiotherapy, surgical excision or combined therapy. However, new therapeutic approaches are required due to the possible recurrence or progression of meningiomas despite initial therapy. Adjunctive therapy with synthetic long-acting somatostatin (SST) analogues has been described in humans with SST-expressing tumours. The expression of SST receptors (SSTRs) by feline meningiomas is currently unknown, and there are little data about canine meningiomas. We hypothesized that SSTR is expressed by canine and feline meningiomas (S1).Methods: Seven canines and 11 felines with histologically confirmed meningiomas underwent STTR screening. RNA expressions of SSTR1, SSTR2, SSTR3 and SSTR5 (canine) and SSTR1-SSTR 5 (feline) in fresh frozen and formalin-fixed and paraffin-embedded (FFPE) samples were investigated using real-time (RT)-qPCR. The expression of SSTR1 and SSTR2 in FFPE samples was evaluated using immunohistochemistry (IHC). The specificity of applied antibodies for canine and feline species was confirmed by western blotting.Results: In canine meningiomas (n = 7), RNA expression of SSTR1, SSTR2 and SSTR5 was detected in all samples; SSTR3 RNA expression was detected in only 33% of samples. In feline meningiomas (n = 12), RNA expression of SSTR1, SSTR4, SSTR5 and SSTR2 was detected in 91%, 46%, 46% and 36% of samples, respectively; SSTR3 was not expressed. Overall, the detection rate was lower in FFPE samples. IHC revealed the expression of SSTR1 and SSTR2 in all samples from both species. However, it is important to exercise caution when interpreting IHC results due to the presence of diffuse background staining.Conclusions: SSTRs are widely expressed in canine and feline meningiomas, thereby encouraging further studies investigating SSTR expression to conduct trials about the effect of adjunctive therapy with long-acting SST-analogues.","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"10 4","pages":"e1537"},"PeriodicalIF":1.8000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11250153/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Medicine and Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/vms3.1537","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: The standard treatment for canine and feline meningiomas includes radiotherapy, surgical excision or combined therapy. However, new therapeutic approaches are required due to the possible recurrence or progression of meningiomas despite initial therapy. Adjunctive therapy with synthetic long-acting somatostatin (SST) analogues has been described in humans with SST-expressing tumours. The expression of SST receptors (SSTRs) by feline meningiomas is currently unknown, and there are little data about canine meningiomas. We hypothesized that SSTR is expressed by canine and feline meningiomas (S1).

Methods: Seven canines and 11 felines with histologically confirmed meningiomas underwent STTR screening. RNA expressions of SSTR1, SSTR2, SSTR3 and SSTR5 (canine) and SSTR1-SSTR 5 (feline) in fresh frozen and formalin-fixed and paraffin-embedded (FFPE) samples were investigated using real-time (RT)-qPCR. The expression of SSTR1 and SSTR2 in FFPE samples was evaluated using immunohistochemistry (IHC). The specificity of applied antibodies for canine and feline species was confirmed by western blotting.

Results: In canine meningiomas (n = 7), RNA expression of SSTR1, SSTR2 and SSTR5 was detected in all samples; SSTR3 RNA expression was detected in only 33% of samples. In feline meningiomas (n = 12), RNA expression of SSTR1, SSTR4, SSTR5 and SSTR2 was detected in 91%, 46%, 46% and 36% of samples, respectively; SSTR3 was not expressed. Overall, the detection rate was lower in FFPE samples. IHC revealed the expression of SSTR1 and SSTR2 in all samples from both species. However, it is important to exercise caution when interpreting IHC results due to the presence of diffuse background staining.

Conclusions: SSTRs are widely expressed in canine and feline meningiomas, thereby encouraging further studies investigating SSTR expression to conduct trials about the effect of adjunctive therapy with long-acting SST-analogues.

查看原文本刊更多论文

犬和猫脑膜瘤中体生长抑素受体的表达。

目标：犬猫脑膜瘤的标准治疗方法包括放射治疗、手术切除或综合治疗。然而，由于脑膜瘤在初始治疗后仍有可能复发或恶化，因此需要新的治疗方法。合成长效体生长抑素（SST）类似物的辅助治疗已在表达 SST 的人类肿瘤中得到应用。目前尚不清楚猫脑膜瘤的 SST 受体（SSTR）表达情况，犬脑膜瘤的相关数据也很少。我们假设犬科和猫科脑膜瘤均表达 SSTR（S1）：方法：对组织学确诊为脑膜瘤的 7 只犬科动物和 11 只猫科动物进行了 STTR 筛查。采用实时（RT）-qPCR 技术检测了新鲜冷冻样本和福尔马林固定及石蜡包埋样本（FFPE）中 SSTR1、SSTR2、SSTR3 和 SSTR5（犬）以及 SSTR1-SSTR 5（猫）的 RNA 表达。使用免疫组织化学（IHC）评估了 FFPE 样品中 SSTR1 和 SSTR2 的表达。通过免疫印迹法确认了所应用的抗体对犬科和猫科动物的特异性：结果：在犬脑膜瘤（n = 7）中，所有样本均检测到 SSTR1、SSTR2 和 SSTR5 的 RNA 表达；仅 33% 的样本检测到 SSTR3 RNA 表达。在猫脑膜瘤（n = 12）中，分别有 91%、46%、46% 和 36% 的样本检测到 SSTR1、SSTR4、SSTR5 和 SSTR2 的 RNA 表达；SSTR3 没有表达。总体而言，FFPE样本的检出率较低。IHC 检测显示，SSTR1 和 SSTR2 在两个物种的所有样本中均有表达。然而，由于存在弥漫性背景染色，在解释 IHC 结果时必须谨慎：结论：SSTRs在犬猫脑膜瘤中广泛表达，因此鼓励进一步研究SSTR的表达，以开展有关长效SST类似物辅助治疗效果的试验。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary Medicine and Science Veterinary-General Veterinary

CiteScore

3.00

自引率

0.00%

发文量

296

期刊介绍： Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science. Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper. We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell. Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.