Low-oxygen-induced root bending is altered by phytoglobin1 through mediation of ethylene response factors (ERFs) and auxin signaling.

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-07-16 DOI:10.1007/s00425-024-04482-3
Mohammed M Mira, Robert D Hill, Claudio Stasolla
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引用次数: 0

Abstract

Main conclusion: phytoglobin1 positively regulates root bending in hypoxic Arabidopsis roots through regulation of ethylene response factors and auxin transport. Hypoxia-induced root bending is known to be mediated by the redundant activity of the group VII ethylene response factors (ERFVII) RAP2.12 and HRE2, causing changes in polar auxin transport (PAT). Here, we show that phytoglobin1 (Pgb1), implicated in hypoxic adaptation through scavenging of nitric oxide (NO), can alter root direction under low oxygen. Hypoxia-induced bending is exaggerated in roots over-expressing Pgb1 and attenuated in those where the gene is suppressed. These effects were attributed to Pgb1 repressing both RAP2.12 and HRE2. Expression, immunological and genetic data place Pgb1 upstream of RAP2.12 and HRE2 in the regulation of root bending in oxygen-limiting environments. The attenuation of slanting in Pgb1-suppressing roots was associated with depletion of auxin activity at the root tip because of depression in PAT, while exaggeration of root bending in Pgb1-over-expressing roots with the retention of auxin activity. Changes in PIN2 distribution patterns, suggestive of redirection of auxin movement during hypoxia, might contribute to the differential root bending responses of the transgenic lines. In the end, Pgb1, by regulating NO levels, controls the expression of 2 ERFVIIs which, in a cascade, modulate PAT and, therefore, root bending.

Abstract Image

植物血红蛋白1通过乙烯反应因子(ERFs)和辅素信号转导改变低氧诱导的根弯曲。
主要结论:植物血红蛋白1通过调控乙烯响应因子和辅素运输积极调节缺氧拟南芥根弯曲。已知缺氧诱导的根弯曲是由第七组乙烯响应因子(ERFVII)RAP2.12 和 HRE2 的冗余活性介导的,从而引起极性辅素运输(PAT)的变化。在这里,我们发现植物血红蛋白1(Pgb1)通过清除一氧化氮(NO)与缺氧适应有关,它能改变根在低氧条件下的方向。缺氧诱导的弯曲在过度表达 Pgb1 的根中会加剧,而在基因被抑制的根中则会减弱。这些效应归因于 Pgb1 同时抑制了 RAP2.12 和 HRE2。表达、免疫学和遗传学数据表明,Pgb1 在限氧环境中调节根弯曲的过程中处于 RAP2.12 和 HRE2 的上游。抑制 Pgb1 的根的倾斜度减弱与 PAT 的抑制导致根尖的辅素活性耗竭有关,而过量表达 Pgb1 的根的根弯曲加剧与辅素活性的保留有关。PIN2 分布模式的变化表明缺氧时辅助素运动方向发生了改变,这可能是转基因品系根弯曲反应不同的原因之一。最后,Pgb1 通过调节氮氧化物水平,控制了 2 个 ERFVII 的表达,这 2 个 ERFVII 以级联的方式调节了 PAT,从而调节了根弯曲。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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