Vancomycin-Loaded Sol–Gel System for In Situ Coating of Artificial Bone to Prevent Surgical Site Infections

IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Xin Cui, Tian-Ci Wei, Lu-Ming Guo, Guo-Yang Xu, Kuo Zhang, Qing-Shi Zhang, Xiong Xu, Gui-Yuan Wang, Litao Li, Hong-Wen Liang, Lei Wang, Xu Cui
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Abstract

Surgical site infections (SSIs) related to implants have always been a major challenge for clinical doctors and patients. Clinically, doctors may directly apply antibiotics into the wound to prevent SSIs. However, this strategy is strongly associated with experience of doctors on the amount and the location of antibiotics. Herein, an in situ constructable sol–gel system is developed containing antibiotics during surgical process and validated the efficacy against SSIs in beagles. The system involves chitosan (CS), β-glycerophosphate (β-GP) and vancomycin (VAN), which can be adsorbed onto porous hydroxyapatite (HA) and form VAN-CS/β-GP@HA hydrogel in a short time. The VAN concentration from VAN-CS/β-GP@HA hydrogel is higher than minimum inhibitory concentration (MIC) against Staphylococcus aureus (S. aureus) at the 21st day in vitro. In an in vivo canine model for the prevention of SSIs in the femoral condyle, VAN-CS/β-GP@HA exhibits excellent biocompatibility, antimicrobial properties, and promotion of bone healing. In all, the CS/β-GP instant sol–gel system is able to in situ encapsulate antibiotics and adhere on artificial bone implants during the surgery, effectively preventing SSIs related to implants.

Abstract Image

用于人工骨原位涂层的万古霉素载体溶胶凝胶系统,可预防手术部位感染。
与植入物相关的手术部位感染(SSI)一直是临床医生和患者面临的一大挑战。在临床上,医生可能会直接在伤口处使用抗生素来预防 SSI。然而,这种策略与医生对抗生素用量和位置的经验密切相关。本文开发了一种在手术过程中含有抗生素的原位可构建溶胶-凝胶系统,并验证了该系统对小猎犬 SSIs 的疗效。该系统包括壳聚糖(CS)、β-甘油磷酸酯(β-GP)和万古霉素(VAN),它们可吸附在多孔羟基磷灰石(HA)上,并在短时间内形成 VAN-CS/β-GP@HA 水凝胶。在体外第 21 天,VAN-CS/β-GP@HA 水凝胶中的 VAN 浓度高于金黄色葡萄球菌(S. aureus)的最小抑菌浓度(MIC)。在预防股骨髁SSI的犬体内模型中,VAN-CS/β-GP@HA表现出了良好的生物相容性、抗菌性和促进骨愈合的特性。总之,CS/β-GP 瞬时溶胶-凝胶系统能够在手术过程中原位封装抗生素并附着在人工骨植入物上,从而有效预防与植入物相关的 SSI。
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来源期刊
Macromolecular bioscience
Macromolecular bioscience 生物-材料科学:生物材料
CiteScore
7.90
自引率
2.20%
发文量
211
审稿时长
1.5 months
期刊介绍: Macromolecular Bioscience is a leading journal at the intersection of polymer and materials sciences with life science and medicine. With an Impact Factor of 2.895 (2018 Journal Impact Factor, Journal Citation Reports (Clarivate Analytics, 2019)), it is currently ranked among the top biomaterials and polymer journals. Macromolecular Bioscience offers an attractive mixture of high-quality Reviews, Feature Articles, Communications, and Full Papers. With average reviewing times below 30 days, publication times of 2.5 months and listing in all major indices, including Medline, Macromolecular Bioscience is the journal of choice for your best contributions at the intersection of polymer and life sciences.
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