Surpassing light inhomogeneities in structured-illumination microscopy with FlexSIM

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-16 DOI:10.1111/jmi.13344

Emmanuel Soubies, Alejandro Nogueron, Florence Pelletier, Thomas Mangeat, Christophe Leterrier, Michael Unser, Daniel Sage

引用次数: 0

Abstract

Super-resolution structured-illumination microscopy (SIM) is a powerful technique that allows one to surpass the diffraction limit by up to a factor two. Yet, its practical use is hampered by its sensitivity to imaging conditions which makes it prone to reconstruction artefacts. In this work, we present FlexSIM, a flexible SIM reconstruction method capable to handle highly challenging data. Specifically, we demonstrate the ability of FlexSIM to deal with the distortion of patterns, the high level of noise encountered in live imaging, as well as out-of-focus fluorescence. Moreover, we show that FlexSIM achieves state-of-the-art performance over a variety of open SIM datasets.

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利用 FlexSIM 解决结构照明显微镜中的光不均匀问题。

超分辨结构照明显微镜（SIM）是一种强大的技术，可使衍射极限提高两倍。然而，它对成像条件的敏感性使其在实际应用中容易产生重建伪影。在这项工作中，我们提出了 FlexSIM，一种能够处理高难度数据的灵活 SIM 重建方法。具体来说，我们展示了 FlexSIM 处理图案失真、现场成像中遇到的高水平噪声以及焦外荧光的能力。此外，我们还展示了 FlexSIM 在各种开放 SIM 数据集上实现的一流性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.