A comprehensive investigation of the anion inhibition profile of a β-carbonic anhydrase from Acinetobacter baumannii for crafting innovative antimicrobial treatments.

IF 5.6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Viviana De Luca, Simone Giovannuzzi, Claudiu T Supuran, Clemente Capasso
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引用次数: 0

Abstract

This study refers to the intricate world of Acinetobacter baumannii, a resilient pathogenic bacterium notorious for its propensity at antibiotic resistance in nosocomial infections. Expanding upon previous findings that emphasised the bifunctional enzyme PaaY, revealing unexpected γ-carbonic anhydrase (CA) activity, our research focuses on a different class of CA identified within the A. baumannii genome, the β-CA, designated as 𝛽-AbauCA (also indicated as CanB), which plays a crucial role in the resistance mechanism mediated by AmpC beta-lactamase. Here, we cloned, expressed, and purified the recombinant 𝛽-AbauCA, unveiling its distinctive kinetic properties and inhibition profile with inorganic anions (classical CA inhibitors). The exploration of 𝛽-AbauCA not only enhances our understanding of the CA repertoire of A. baumannii but also establishes a foundation for targeted therapeutic interventions against this resilient pathogen, promising advancements in combating its adaptability and antibiotic resistance.

全面研究鲍曼不动杆菌β-碳酸酐酶的阴离子抑制谱,以开发创新的抗菌疗法。
本研究涉及鲍曼不动杆菌的复杂世界,鲍曼不动杆菌是一种顽强的致病细菌,因其在医院内感染中的抗生素耐药性倾向而臭名昭著。以前的研究结果强调了双功能酶 PaaY,揭示了意想不到的 γ-碳酸酐酶(CA)活性,在此基础上,我们的研究重点是在鲍曼不动杆菌基因组中发现的另一类 CA--β-CA,即 β-CA,命名为 "绊-AbauCA"(也称 CanB),它在由 AmpC β-内酰胺酶介导的耐药机制中发挥着至关重要的作用。在此,我们克隆、表达并纯化了重组的 β-CbauCA,揭示了其独特的动力学特性以及与无机阴离子(经典 CA 抑制剂)的抑制谱。对 β-AbauCA的探索不仅加深了我们对鲍曼尼氏菌CA剧目的了解,还为针对这种生命力顽强的病原体进行有针对性的治疗干预奠定了基础,有望在抗击其适应性和抗生素耐药性方面取得进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
10.30
自引率
10.70%
发文量
195
审稿时长
4-8 weeks
期刊介绍: Journal of Enzyme Inhibition and Medicinal Chemistry publishes open access research on enzyme inhibitors, inhibitory processes, and agonist/antagonist receptor interactions in the development of medicinal and anti-cancer agents. Journal of Enzyme Inhibition and Medicinal Chemistry aims to provide an international and interdisciplinary platform for the latest findings in enzyme inhibition research. The journal’s focus includes current developments in: Enzymology; Cell biology; Chemical biology; Microbiology; Physiology; Pharmacology leading to drug design; Molecular recognition processes; Distribution and metabolism of biologically active compounds.
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