Phylogeography, Genetic Diversity and Population Structure of Echinococcus granulosus Sensu Stricto Inferred by Mitochondrial DNA Markers between Southeast of Iran and Pakistan.

IF 0.9 4区 医学 Q4 PARASITOLOGY
Davood Anvari, Shirzad Gholami, Adel Spotin, Seyed Abdollah Hosseini, Ahmad Daryani, Shahabeddin Sarvi, Mohammad Reza Narouei, Mona Hosseini, Abolghasem Siyadatpanah
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引用次数: 0

Abstract

Background: Current study was designed to provide a better insight into the circulating genotypes, genetic diversity, and population structure of Echinococcus spp. between southeast of Iran and Pakistan.

Methods: From Jun 2020 to Dec 2020, 46 hydatid cysts were taken from human (n: 6), camel (n: 10), goat (n: 10), cattle (n: 10) and sheep (n: 10) in various cities of Sistan and Baluchestan Province of Iran, located at the neighborhood of Pakistan. DNA samples were extracted, amplified, and subjected to sequence analysis of cox1 and nad1 genes.

Results: The phylogeny inferred by the Maximum Likelihood algorithm indicated that G1 genotype (n: 19), G3 genotype (n: 14) and G6 genotype (n: 13) assigned into their specific clades. The diversity indices showed a moderate (nad1: Hd: 0.485) to high haplotype diversity (cox1: Hd: 0.867) of E. granulosus s.s. (G1/G3) and low nucleotide diversity. The negative value of Tajima's D and Fu's Fs test displayed deviation from neutrality indicating a recent population expansion. A parsimonious network of the haplotypes of cox1 displayed star-like features in the overall population containing IR9/PAK1/G1, IR2/PAK2/G3 and IR18/G6 as the most common haplotypes. A pairwise fixation index (Fst) indicated that E. granulosus s.s. populations are genetically moderate differentiated between southeast of Iran and Pakistan. The extension of haplotypes PAK18/G1 (sheep) and PAK26/G1 (cattle) toward Iranian haplogroup revealed that there is dawn of Echinococcus flow due to a transfer of alleles between mentioned populations through transport of livestock or their domestication.

Conclusion: The current findings strengthen our knowledge concerning the evolutionary paradigms of E. granulosus in southeastern borders of Iran and is effective in controlling of hydatidosis.

通过线粒体 DNA 标记推断伊朗东南部与巴基斯坦之间棘球蚴的系统地理学、遗传多样性和种群结构。
背景:本研究旨在更好地了解伊朗东南部和巴基斯坦之间棘球蚴属的循环基因型、遗传多样性和种群结构:本研究旨在更好地了解伊朗东南部和巴基斯坦之间棘球蚴属的循环基因型、遗传多样性和种群结构:方法:2020 年 6 月至 2020 年 12 月,在位于巴基斯坦邻近地区的伊朗锡斯坦和俾路支斯坦省的多个城市从人(6 个)、骆驼(10 个)、山羊(10 个)、牛(10 个)和绵羊(10 个)身上采集了 46 个包虫囊肿。对 DNA 样本进行提取、扩增,并对 cox1 和 nad1 基因进行序列分析:用最大似然法推断的系统发生表明,G1 基因型(19 个)、G3 基因型(14 个)和 G6 基因型(13 个)被归入各自特定的支系。多样性指数显示,颗粒衣壳虫(G1/G3)具有中等(nad1:Hd:0.485)到较高的单体型多样性(cox1:Hd:0.867),核苷酸多样性较低。Tajima's D 和 Fu's Fs 检验的负值显示偏离了中性,这表明种群最近有所扩大。cox1 单倍型的拟合网络在整个群体中显示出星形特征,其中 IR9/PAK1/G1、IR2/PAK2/G3 和 IR18/G6 是最常见的单倍型。成对固定指数(Fst)表明,E. granulosus s.s.种群在伊朗东南部和巴基斯坦之间的遗传差异适中。单倍型 PAK18/G1(羊)和 PAK26/G1(牛)向伊朗单倍群的延伸表明,由于牲畜的运输或驯化造成的等位基因在上述种群之间的转移,出现了棘球蚴流动的曙光:目前的研究结果加强了我们对伊朗东南边境地区格氏棘球蚴进化模式的了解,并能有效控制包虫病。
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来源期刊
CiteScore
1.80
自引率
0.00%
发文量
55
审稿时长
6-12 weeks
期刊介绍: Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members. The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.
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